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Anti-SP3 Antibody (C-Terminus) IHC-plus™ LS-B1648

Note: This antibody replaces LS-C40917
Catalog Size Price
LS-B1648-50 50 µl Unavailable

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Anti-SP3 Antibody (C-Terminus) IHC-plus™ LS-B1648
Rabbit Polyclonal (IgG) to Human SP3
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SP3 Antibody (aa287-336) IHC-plus™ LS-B3502
Rabbit Polyclonal (IgG) to Human SP3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
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Anti-SP3 Antibody (Internal) LS-C137089
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Anti-SP3 Antibody (aa1-74) LS-C311912
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Anti-SP3 Antibody IHC-plus™ LS-B11770
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100% Guaranteed
Rabbit Polyclonal (IgG) to Human SP3
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human SP3
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Western blot

Specificity and Use

SP3 antibody was raised against synthetic peptide from human SP3.
C-Terminus
Peptide mapping to the carboxy terminus of human Sp3
Immunohistochemistry: LS-B1648 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1648 was determined to be 1:50.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 0.2% gelatin.
+4°C, avoid freezing.
For research use only.

About SP3

Q02447 X68560 CAA48562.1

SP3 Antibody, SPR2 Antibody, Transcription factor Sp3 Antibody, Sp3 transcription factor Antibody, SPR-2 Antibody, Specificity protein 3 Antibody

SP3 belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes.

Immunohistochemistry

Immunohistochemistry
Anti-SP3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1648 dilution 1:50.

Western blot

Western blot
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Sp3 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

Requested From: 
Date Requested: 12/15/2017

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