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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SP2 Antibody (aa28‑77) LS‑C109775
SP2 antibody LS-C109775 is an unconjugated rabbit polyclonal antibody to SP2 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C109775-50
50 µg
Unavailable

Popular SP2 Products

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Anti-SP2 antibody IHC staining of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10251 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Antibody
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Applications: Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA

Product Description

SP2 antibody LS-C109775 is an unconjugated rabbit polyclonal antibody to SP2 from human, mouse, rat and other species. Validated for WB.
About SP2
SP2 is a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. Q02086 NM_003110 NP_003101.3

SP2 Antibody, KIAA0048 Antibody, Sp2 transcription factor Antibody, Transcription factor Sp2 Antibody


Specifications

Target
Human SP2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Rabbit, Chicken, Drosophila (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
SP2 antibody was raised against synthetic peptide located between aa28-77 of human SP2 (Q02086). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Turkey, Chicken, Drosophila (100%); Lizard (91%).
Blocking Peptide
LS-E11755 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109775. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa28-77
Specificity
Human SP2
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

SP2 antibody LS-C109775 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SP2 antibody LS-C109775 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SP2 antibody LS-C109775 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SP2 antibody LS-C109775 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SP2 antibody LS-C109775 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SP2 antibody LS-C109775 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SP2 antibody LS-C109775 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SP2 antibody LS-C109775 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/17/2018

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