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Catalog Number Size Price
LS-C161409-400 400 µl $393 
SP140 Antibody - SP140 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SP140 Antibody - Fluorescent confocal image of HeLa cell stained with SP140 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SP140 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SP140 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
SP140 Antibody - SP140 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The SP140 antibody detected the SP140 protein (arrow).
SP140 Antibody - SP140 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SP140 Antibody - Fluorescent confocal image of HeLa cell stained with SP140 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SP140 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SP140 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
SP140 Antibody - SP140 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The SP140 antibody detected the SP140 protein (arrow).
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Polyclonal Rabbit anti‑Human SP140 Antibody (aa272‑301, IHC, IF, WB) LS‑C161409

Polyclonal Rabbit anti‑Human SP140 Antibody (aa272‑301, IHC, IF, WB) LS‑C161409

Antibody:
SP140 Rabbit anti-Human Polyclonal (aa272-301) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C161409-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SP140 Rabbit anti-Human Polyclonal (aa272-301) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SP140 antibody LS-C161409 is an unconjugated rabbit polyclonal antibody to human SP140 (aa272-301). Validated for IF, IHC and WB.
Target
Human SP140
Synonyms
SP140 | Nuclear autoantigen Sp-140 | Speckled 140 kDa | LYSP100 | SP140 nuclear body protein | LYSP100-A | LYSP100-B | Nuclear body protein SP140
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa272-301
Specificity
This SP140 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-301 amino acids from the Central region of human SP140.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SP140
Q13342 NM_007237 NP_009168.4

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024