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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SP1 Antibody (clone 1A5) IHC‑plus™ LS‑B6148
Note: This antibody replaces LS-C133445
SP1 antibody LS-B6148 is an unconjugated mouse monoclonal antibody to SP1 from human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6148-50
50 µg (0.43 mg/ml)
Unavailable

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Western blot of extracts from COS7 cells treated with serum 20% 15', using SP1 (Phospho-Thr739) Antibody. The lane on the right is treated with the synthesized peptide.
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Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]
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Product Description

SP1 antibody LS-B6148 is an unconjugated mouse monoclonal antibody to SP1 from human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About SP1
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). P08047 NM_138473 NP_612482.2

SP1 Antibody, Sp1 transcription factor Antibody, Transcription factor Sp1 Antibody, Specificity protein 1 Antibody, TSFP1 Antibody


Specifications

Target
Human SP1
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [1A5]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SP1 antibody was raised against sP1 (NP_612482, 89 a.a. ~ 199 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human SP1
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4.
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-SP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6148 concentration 5 ug/ml.
Anti-SP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6148 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

SP1 monoclonal antibody (M02), clone 1A5 Western blot of SP1 expression in HeLa NE.
SP1 monoclonal antibody (M02), clone 1A5 Western blot of SP1 expression in HeLa NE.

Western blot

SP1 monoclonal antibody (M02), clone 1A5. Western blot of SP1 expression in NIH/3T3.
SP1 monoclonal antibody (M02), clone 1A5. Western blot of SP1 expression in NIH/3T3.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SP1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SP1 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-SP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6148 concentration 5 ug/ml.
Anti-SP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6148 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

SP1 monoclonal antibody (M02), clone 1A5 Western blot of SP1 expression in HeLa NE.
SP1 monoclonal antibody (M02), clone 1A5 Western blot of SP1 expression in HeLa NE.

Western blot

SP1 monoclonal antibody (M02), clone 1A5. Western blot of SP1 expression in NIH/3T3.
SP1 monoclonal antibody (M02), clone 1A5. Western blot of SP1 expression in NIH/3T3.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SP1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SP1 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-SP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6148 concentration 5 ug/ml.
Anti-SP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6148 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

SP1 monoclonal antibody (M02), clone 1A5 Western blot of SP1 expression in HeLa NE.
SP1 monoclonal antibody (M02), clone 1A5 Western blot of SP1 expression in HeLa NE.

Western blot

SP1 monoclonal antibody (M02), clone 1A5. Western blot of SP1 expression in NIH/3T3.
SP1 monoclonal antibody (M02), clone 1A5. Western blot of SP1 expression in NIH/3T3.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SP1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SP1 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-SP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6148 concentration 5 ug/ml.
Anti-SP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6148 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

SP1 monoclonal antibody (M02), clone 1A5 Western blot of SP1 expression in HeLa NE.
SP1 monoclonal antibody (M02), clone 1A5 Western blot of SP1 expression in HeLa NE.

Western blot

SP1 monoclonal antibody (M02), clone 1A5. Western blot of SP1 expression in NIH/3T3.
SP1 monoclonal antibody (M02), clone 1A5. Western blot of SP1 expression in NIH/3T3.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SP1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SP1 is approximately 0.1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/23/2018

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