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Anti-SP1 Antibody (clone 1A5) IHC-plus™ LS-B6148

Note: This antibody replaces LS-C133445

Ordering

Wt. Vol. Conc. Price
50 µg - 0.43 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SP1 Antibodies

Anti-SP1 Antibody (Internal) IHC-plus™ LS-B1647
Rabbit Polyclonal (IgG) to Human SP1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SP1 Antibody (clone 1A5) IHC-plus™ LS-B6148
Mouse Monoclonal [clone 1A5] (IgG1) to Human SP1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SP1 Antibody (aa735-785) LS-C290594
Goat Polyclonal to Human SP1
Human, Mouse, Bovine, Dog, Goat, Horse, Chicken, Xenopus
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1A5] (IgG1) to Human SP1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human SP1
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1A5]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA

Specificity and Use

SP1 antibody was raised against sP1 (NP_612482, 89 a.a. ~ 199 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human SP1
Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Recombinant protein.

Packaging

PBS, pH 7.4.
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About SP1

P08047 NM_138473 NP_612482.2

SP1 Antibody, Sp1 transcription factor Antibody, Transcription factor Sp1 Antibody, Specificity protein 1 Antibody, TSFP1 Antibody

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation).

Immunohistochemistry

Immunohistochemistry
Anti-SP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6148 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
SP1 monoclonal antibody (M02), clone 1A5 Western blot of SP1 expression in HeLa NE.

Western blot

Western blot
SP1 monoclonal antibody (M02), clone 1A5. Western blot of SP1 expression in NIH/3T3.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged SP1 is approximately 0.1 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/6/2016

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