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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SOX3 Antibody (aa413‑427) IHC‑plus™ LS‑B8332
Note: This antibody replaces LS-C113021
SOX3 antibody LS-B8332 is an unconjugated goat polyclonal antibody to SOX3 from human, mouse, monkey and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B8332-50
50 µg (0.5 mg/ml)
Unavailable

Popular SOX3 Products

Antibody
Species: Human, Rodent
Applications: IHC, IHC - Frozen, Western blot, ELISA
MCF7 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Dog, Guinea pig, Pig, Rabbit
Applications: Western blot
Anti-SOX3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1569 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Dog, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
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Species: Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Rabbit
Applications: Western blot

Product Description

SOX3 antibody LS-B8332 is an unconjugated goat polyclonal antibody to SOX3 from human, mouse, monkey and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About SOX3
Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. P41225 NM_005634 NP_005625.2

SOX3 Antibody, GHDX Antibody, MRGH Antibody, SOXB Antibody, Panhypopituitarism Antibody, PHPX Antibody, Sry-box3 Antibody, PHP Antibody, Transcription factor SOX-3 Antibody


Specifications

Target
Human SOX3
Host
Goat
Reactivity
Human, Monkey, Mouse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat, Dog (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.5 - 1.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SOX3 antibody was raised against synthetic peptide C-DAASPLPGGRLHGVH from the C-terminus of human SOX3 (NP_005625.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rabbit (100%); Bat, Dog (93%); Elephant (80%).
Blocking Peptide
LS-E27374 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8332. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa413-427
Specificity
Human SOX3.
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 42kDa band observed in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates (calculated MW of 45.2kDa according to NP_005625.2). Recommended concentration: 0.5-1.5 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-SOX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8332 dilution 3.75 ug/ml.
Anti-SOX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8332 dilution 3.75 ug/ml.

Western blot

SOX3 antibody (0.5 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SOX3 antibody (0.5 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-SOX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8332 dilution 3.75 ug/ml.
Anti-SOX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8332 dilution 3.75 ug/ml.

Western blot

SOX3 antibody (0.5 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SOX3 antibody (0.5 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-SOX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8332 dilution 3.75 ug/ml.
Anti-SOX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8332 dilution 3.75 ug/ml.

Western blot

SOX3 antibody (0.5 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SOX3 antibody (0.5 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-SOX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8332 dilution 3.75 ug/ml.
Anti-SOX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8332 dilution 3.75 ug/ml.

Western blot

SOX3 antibody (0.5 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SOX3 antibody (0.5 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/19/2018

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