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Anti-SOX15 Antibody LS-C39197

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $635
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular SOX15 Antibodies

Anti-SOX15 Antibody (aa95-125, APC) LS-C269990
Rabbit Polyclonal (IgG) to Human SOX15
Human
IHC, Western blot, ELISA
APC Conjugated
Anti-SOX15 Antibody (aa95-125, FITC) LS-C269992
Rabbit Polyclonal (IgG) to Human SOX15
Human
IHC, Western blot, ELISA
FITC Conjugated
Anti-SOX15 Antibody (aa95-125, Azide-free, HRP) LS-C269993
Rabbit Polyclonal (IgG) to Human SOX15
Human
IHC, Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human SOX15
Human
ICC, Western blot, ELISA
Unconjugated

Details

Human SOX15
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC
  • Western blot
  • ELISA

Specificity and Use

SOX15 antibody was raised against e. coli-derived rhSOX15.
Selected for its ability to recognize human SOX15 in the applications listed.
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 20 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows less than 1% cross-reactivity with rhSOX1, -2, -5, -6, -7, -10, -12, -14, -15, -17, and rhSOX21. Immunocytochemistry: This antibody can be used at a concentration of 10 ug/ml to detect SOX15 in NTera2 teratocarcinoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 0.3 ng/well.

Packaging

Lyophilized.
100 µl deionized water
For research use only.

About SOX15

O60248 NM_006942 AAH00985.1

SOX15 Antibody, Protein SOX-15 Antibody, Protein SOX-20 Antibody, SOX26 Antibody, SOX20 Antibody, Protein SOX-12 Antibody, SOX27 Antibody

SOX15 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins.

Requested From: United States
Date Requested: 12/9/2016

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