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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SOS1 Antibody (aa1243‑1258) LS‑C10294
SOS1 antibody LS-C10294 is an unconjugated rabbit polyclonal antibody to SOS1 from human, mouse, dog and other species. Validated for IP and WB. Cited in 1 publication.
Antibody
Catalog
Size
Price
LS-C10294-200
200 µg (1 mg/ml)
$635

Popular SOS1 Products

Antibody
Species: Human, Monkey, Mouse, Bat, Bovine, Horse, Pig, Rabbit
Applications: Immunofluorescence, Western blot, ELISA
Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit
Applications: Immunofluorescence, Western blot, ELISA
Human Colon, Submucosal Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA

Product Description

SOS1 antibody LS-C10294 is an unconjugated rabbit polyclonal antibody to SOS1 from human, mouse, dog and other species. Validated for IP and WB. Cited in 1 publication.

Specifications

Target
Human SOS1
Synonyms
SOS1, GINGF, NS4, SOS-1, Son of sevenless homolog 1, GF1, GGF1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Dog, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat, Bovine, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 1 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
SOS1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 1243-1258 (KKSDHGNAFFPNSPSP) of murine Son of Sevenless Homolog 1 Protein (SOS 1). The immunizing sequence is identical in human. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Horse, Rabbit (100%); Bovine, Bat, Pig (94%); Opossum (88%).
Epitope
aa1243-1258
Specificity
Recognizes SOS 1, Mr 175kD. Species cross-reactivity: Mouse and human. Other species not tested.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-1 ug/ml. Immunoprecipitation: 5 ug. Positive control: F4209: Fibroblast Cell, 3T3/A31. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
Presentation
0.1 M Tris, pH 8.0, 0.15 M sodium chloride, 0.1 mM EDTA, 0.05% sodium azide, 30% glycerol.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SOS1
Promotes the exchange of Ras-bound GDP by GTP. Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity. Q07889 NM_005633 NP_005624.2


Publications (1)

1
Lentiviral vector-based insertional mutagenesis identifies genes associated with liver cancer. Ranzani M, Cesana D, Bartholomae CC, Sanvito F, Pala M, Benedicenti F, Gallina P, Sergi LS, Merella S, Bulfone A, Doglioni C, von Kalle C, Kim YJ, Schmidt M, Tonon G, Naldini L, Montini E. Nature methods. 2013 10:155-61. (WB; Human) [Full Text Article] [PubMed:23314173] [PMC:PMC3589714]

Customer Reviews (0)


Requested From: United States
Date Requested: 10/20/2018

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