Human, Monkey, Mouse, Dog, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Bat, Bovine, Pig (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (0.5 - 1 µg/ml)
- (applications tested for the base form of this product only)
SOS1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 1243-1258 (KKSDHGNAFFPNSPSP) of murine Son of Sevenless Homolog 1 Protein (SOS 1). The immunizing sequence is identical in human. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Horse, Rabbit (100%); Bovine, Bat, Pig (94%); Opossum (88%).
Recognizes SOS 1, Mr 175kD. Species cross-reactivity: Mouse and human. Other species not tested.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-1 ug/ml. Immunoprecipitation: 5 ug. Positive control: F4209: Fibroblast Cell, 3T3/A31. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
0.1 M Tris, pH 8.0, 0.15 M sodium chloride, 0.1 mM EDTA, 0.05% sodium azide, 30% glycerol.
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.