Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
SOS1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 1243-1258 (KKSDHGNAFFPNSPSP) of murine Son of Sevenless Homolog 1 Protein (SOS 1). The immunizing sequence is identical in human. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Horse, Rabbit (100%); Bovine, Bat, Pig (94%); Opossum (88%).
Recognizes SOS 1, Mr 175kD. Species cross-reactivity: Mouse and human. Other species not tested.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-1 ug/ml. Immunoprecipitation: 5 ug. Positive control: F4209: Fibroblast Cell, 3T3/A31. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
0.1 M Tris, pH 8.0, 0.15 M sodium chloride, 0.1 mM EDTA, 0.05% sodium azide, 30% glycerol.
Promotes the exchange of Ras-bound GDP by GTP. Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity.