Ponsin antibody LS-B1762 is an unconjugated goat polyclonal antibody to Ponsin (SORBS1) from human, mouse, rat and other species. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Human SORBS1 / Ponsin
Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse
(tested or 100% immunogen sequence identity)
Pig, Chicken (at least 90% immunogen sequence identity)
- IHC - Paraffin (2.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
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SORBS1 / Ponsin antibody was raised against synthetic peptide C-GTFPGNYVKPLYL from the C-terminus of human SORBS1 (NP_006425.2; NP_056200.1; NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_079267.1; NP_001030129.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Hamster, Elephant, Panda, Horse, Opossum, Platypus (100%); Pig, Turkey, Chicken (92%); Zebrafish (85%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1762. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human SORBS1. This antibody is expected to recognize all seven reported isoforms (NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1 and NP_001030129.1).
Immunohistochemistry: LS-B1762 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1762 was determined to be 2.5 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.