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Antibodies > SOD2 / Mn SOD > LS-C392600
 
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Catalog Number Size Price
LS-C392600-200 200 µl $393 
SOD2 / Mn SOD Antibody - Antibody staining SODM in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
SOD2 / Mn SOD Antibody - Antibody staining SODM in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
SOD2 / Mn SOD Antibody - All lanes: Anti-SODM Antibody at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: HeLa whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
SOD2 / Mn SOD Antibody - Overlay histogram showing A549 cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
SOD2 / Mn SOD Antibody - Antibody staining SODM in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
SOD2 / Mn SOD Antibody - Antibody staining SODM in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
SOD2 / Mn SOD Antibody - All lanes: Anti-SODM Antibody at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: HeLa whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
SOD2 / Mn SOD Antibody - Overlay histogram showing A549 cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Monoclonal Mouse anti‑Human SOD2 / Mn SOD Antibody (clone 1559CT572.3.83, IHC, WB) LS‑C392600

Monoclonal Mouse anti‑Human SOD2 / Mn SOD Antibody (clone 1559CT572.3.83, IHC, WB) LS‑C392600

Submit Review LSBio Guarantee
Antibody:
SOD2 / Mn SOD Mouse anti-Human Monoclonal (1559CT572.3.83) Antibody
Application:
IHC, IHC-P, WB, Flo, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C392600-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SOD2 / Mn SOD Mouse anti-Human Monoclonal (1559CT572.3.83) Antibody
Application:
IHC, IHC-P, WB, Flo, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Mn SOD antibody LS-C392600 is an unconjugated mouse monoclonal antibody to Mn SOD (SOD2) from human. It is reactive with human and mouse. Validated for Flow, IHC, Peptide-ELISA and WB.
Target
Human SOD2 / Mn SOD
Synonyms
SOD2 | IPOB | MVCD6 | Indophenoloxidase B | Mn superoxide dismutase | MNSOD | Mangano-superoxide dismutase
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1559CT572.3.83
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SOD2 / Mn SOD
P04179 NM_000636 NP_000627.2

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SOD2 / Mn SOD Antibody - Anti-SOD2 / MNSOD antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ SOD2 / Mn SOD Antibody LS‑B3694
Species: Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Xenopus, Drosophila
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
SOD2 / Mn SOD Antibody - Anti-SOD2 / MNSOD antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
PathPlus™ SOD2 / Mn SOD Antibody LS‑B3161
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
SOD2 / Mn SOD Antibody - Anti-MNSOD / SOD2 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
PathPlus™ SOD2 / Mn SOD Antibody LS‑B9291
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Western blot
SOD2 / Mn SOD Antibody - Anti-MNSOD / SOD2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PathPlus™ SOD2 / Mn SOD Antibody LS‑B8169
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus, Gerbil
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Product
SOD2 / Mn SOD Antibody LS‑C39328
Species: Human, Mouse, Rat
Applications: IHC, IHC - Frozen, ICC, Western blot
See all SOD2 / Mn SOD Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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