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Catalog Number Size Price
LS-C407655-10 10 µg $318 
LS-C407655-100 100 µg $470 
SOD2 / Mn SOD Antibody - IHC analysis of SOD2 using anti-SOD2 antibody. SOD2 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SOD2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SOD2 / Mn SOD Antibody - IHC analysis of SOD2 using anti-SOD2 antibody. SOD2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SOD2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SOD2 / Mn SOD Antibody - SOD2 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
SOD2 / Mn SOD Antibody - SOD2 antibody Western blot. All lanes: Anti SOD2 at 0.5 ug/ml. Lane 1: Mouse Testis Tissue Lysate at 50 ug. Lane 2: Mouse Lung Tissue Lysate at 50 ug. Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50 ug. Lane 4: Mouse Liver Tissue Lysate at 50 ug. Lane 5: HEPG2 Whole Cell Lysate at 40 ug. Predicted band size: 25 kD. Observed band size: 25 kD.
SOD2 / Mn SOD Antibody - IHC analysis of SOD2 using anti-SOD2 antibody. SOD2 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SOD2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SOD2 / Mn SOD Antibody - IHC analysis of SOD2 using anti-SOD2 antibody. SOD2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SOD2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SOD2 / Mn SOD Antibody - SOD2 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
SOD2 / Mn SOD Antibody - SOD2 antibody Western blot. All lanes: Anti SOD2 at 0.5 ug/ml. Lane 1: Mouse Testis Tissue Lysate at 50 ug. Lane 2: Mouse Lung Tissue Lysate at 50 ug. Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50 ug. Lane 4: Mouse Liver Tissue Lysate at 50 ug. Lane 5: HEPG2 Whole Cell Lysate at 40 ug. Predicted band size: 25 kD. Observed band size: 25 kD.
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Polyclonal Rabbit anti‑Human SOD2 / Mn SOD Antibody (aa192‑222, IHC, WB) LS‑C407655

Polyclonal Rabbit anti‑Human SOD2 / Mn SOD Antibody (aa192‑222, IHC, WB) LS‑C407655

Antibody:
SOD2 / Mn SOD Rabbit anti-Human Polyclonal (aa192-222) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407655-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SOD2 / Mn SOD Rabbit anti-Human Polyclonal (aa192-222) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
Mn SOD antibody LS-C407655 is an unconjugated rabbit polyclonal antibody to Mn SOD (SOD2) (aa192-222) from human. It is reactive with human, mouse and dog. Validated for IHC and WB.
Target
Human SOD2 / Mn SOD
Synonyms
SOD2 | IPOB | MVCD6 | Indophenoloxidase B | Mn superoxide dismutase | MNSOD | Mangano-superoxide dismutase
Host
Rabbit
Reactivity
Human, Mouse, Dog (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human SOD2 (192-222 aa QYKNVRPDYLKAIWNVINWENVTERYMACKK), different from the related mouse sequence by one amino acid, and from the related rat sequence by four amino acids.
Epitope
aa192-222
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
WB: The detection limit for SOD2 is approximately 0.1 ng/lane under reducing conditions. IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SOD2 / Mn SOD
P04179 NM_000636 NP_000627.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024