Target
Human SOCS7 / SOCS-7
Synonyms
SOCS7 | NAP-4 | Nck-associated protein 4 | SOCS-7 | NAP4 | NCKAP4
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DAGRKPKLTRTQS, from the internal region of the protein sequence according to NP_055413.1.
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 60kD band observed in lysates of Daudi (calculated MW of 63.0kD according to NP_055413.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 28kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee