(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (10 µg/ml)
- Western blot
- ELISA (1:100 - 1:500)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
SOCS3 antibody was raised against full - length SOCS3 protein.
Recognizes suppressor of cytokine signaling 3 (SOCS3), a 30kD member of the suppressor of cytokine signaling family (SOCS) which negatively regulate Janus Kinase (JAK) and signal transducer and activators of transcription (STAT) signaling pathways. SOCS3 specifically acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3. The expression of SOCS3 is inducible by numerous factors, including IL-6, IL-10, Toll-like receptor (TLR) agonists and IFN gamma, making this protein the focus of research into inflammatory diseases, such as Arthritis, Colitis, and Crohn's Disease. SOCS3 expression has also been shown to affect IFN alpha sensitivity in tumor studies.
Immunohistochemistry: LS-B3317 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3317 was determined to be 10 ug/ml. Western Blot: LS-B3317 detects a band of approximately 30kD in Hela cell lysates.
PBS, pH 7.4, 0.09% sodium azide. Sourced in TCS.
4°C or -20°C, Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.