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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SOCS3 Antibody (clone SO1) IHC‑plus™ LS‑B3317
Note: This antibody replaces LS-C44768, LS-C10275
SOCS3 antibody LS-B3317 is an unconjugated mouse monoclonal antibody to human SOCS3. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3317-50
50 µg (1 mg/ml)
Unavailable

Popular SOCS3 Products

Antibody staining (5 ug/ml) of MOLT-4 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Pig, Rabbit
Applications: Western blot
Anti-SOCS3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Western blot of recombinant SOCS3.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot
Western blot analysis of SOCS3 expression in MOLT4 (A); A431 (B); MCF7 (C); Raw264.7 (D); H9C2 (E) whole cell lysates.
Species: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Chicken
Applications: Western blot

Product Description

SOCS3 antibody LS-B3317 is an unconjugated mouse monoclonal antibody to human SOCS3. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About SOCS3
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. O14543 NM_003955 NP_003946.3

SOCS3 Antibody, Cish3 Antibody, Cytokine-induced SH2 protein 3 Antibody, CIS-3 Antibody, CIS3 Antibody, SSI-3 Antibody, STAT-induced STAT inhibitor 3 Antibody, SSI3 Antibody, ATOD4 Antibody, SOCS-3 Antibody


Specifications

Target
Human SOCS3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [SO1]
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • ELISA (1:100 - 1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SOCS3 antibody was raised against full - length SOCS3 protein.
Specificity
Recognizes suppressor of cytokine signaling 3 (SOCS3), a 30kD member of the suppressor of cytokine signaling family (SOCS) which negatively regulate Janus Kinase (JAK) and signal transducer and activators of transcription (STAT) signaling pathways. SOCS3 specifically acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3. The expression of SOCS3 is inducible by numerous factors, including IL-6, IL-10, Toll-like receptor (TLR) agonists and IFN gamma, making this protein the focus of research into inflammatory diseases, such as Arthritis, Colitis, and Crohn's Disease. SOCS3 expression has also been shown to affect IFN alpha sensitivity in tumor studies.
Usage
Immunohistochemistry: LS-B3317 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3317 was determined to be 10 ug/ml. Western Blot: LS-B3317 detects a band of approximately 30kD in Hela cell lysates.
Presentation
PBS, pH 7.4, 0.09% sodium azide. Sourced in TCS.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-SOCS3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3317 concentration 10 ug/ml.
Anti-SOCS3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3317 concentration 10 ug/ml.

Immunohistochemistry

Anti-SOCS3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3317 concentration 10 ug/ml.
Anti-SOCS3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3317 concentration 10 ug/ml.

Immunohistochemistry

Anti-SOCS3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3317 concentration 10 ug/ml.
Anti-SOCS3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3317 concentration 10 ug/ml.

Immunohistochemistry

Anti-SOCS3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3317 concentration 10 ug/ml.
Anti-SOCS3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3317 concentration 10 ug/ml.


Requested From: 
Date Requested: 6/25/2018

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