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Anti-SNCA / Alpha-Synuclein Antibody (aa1-140, clone 5C2) IHC-plus™ LS-B2923

Note: This antibody replaces LS-C93769

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SNCA / Alpha-Synuclein Antibodies

Anti-SNCA / Alpha-Synuclein Antibody (phospho-Ser129) LS-C153370
Rabbit Polyclonal to Human SNCA / Alpha-Synuclein
Human, Mouse, Rat
Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-SNCA / Alpha-Synuclein Antibody (phospho-Ser87) LS-C153371
Rabbit Polyclonal to Human SNCA / Alpha-Synuclein
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Anti-SNCA / Alpha-Synuclein Antibody (HRP) LS-C212005
Rabbit Polyclonal (IgG) to Human SNCA / Alpha-Synuclein
Human
ELISA
HRP Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5C2] (IgG1,k) to Human SNCA / Alpha-Synuclein
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SNCA / Alpha-Synuclein
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [5C2]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA

Specificity and Use

SNCA / Alpha-Synuclein antibody was raised against recombinant human kappa-synuclein (1-140) purified from E. coli.
aa1-140
NAC domain of alpha-synuclein (aa 61-95)
Immunohistochemistry: LS-B2923 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2923 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About SNCA / Alpha-Synuclein

P37840 NM_000345 NP_000336.1

SNCA Antibody, Alpha-synuclein Antibody, PARK4 Antibody, PD1 Antibody, Synuclein alpha-140 Antibody, NACP Antibody, PARK1 Antibody

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease.

Immunohistochemistry

Immunohistochemistry
Anti-SNCA / Alpha-Synuclein antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2923 concentration 10 ug/ml.

Western blot

Western blot
The recombinant human synuclein family (a-, beta-and ?-) and a-synuclein domains (1-60, 1-95, 61-140 and 96-140) proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-a-Synuclein (61-95 aa) antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Requested From: United States
Date Requested: 12/8/2016

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