LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-SNAP25 Antibody (clone SP12) IHC-plus™ LS-B3684

Note: This antibody replaces LS-C11536

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SNAP25 Antibodies

Anti-SNAP25 Antibody (aa183-197, clone B371M) LS-C124563
Mouse Monoclonal [clone B371M] (IgG) to Human SNAP25
Human
IHC, ELISA
Unconjugated
Anti-SNAP25 Antibody (aa183-197, clone 9L651) LS-C124564
Mouse Monoclonal [clone 9L651] (IgG) to Human SNAP25
Human, Rat
IHC, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone SP12] (IgG1) to Human SNAP25
Human, Rat, Hamster, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SNAP25
Mouse
Human, Rat, Hamster, Pig (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [SP12]
Unconjugated
Tissue culture supernatant
Unmodified

Applications

  • IHC - Paraffin (0.2 - 0.5 µg/ml)
  • Western blot (1:500 - 1:5000)
  • ELISA

Specificity and Use

SNAP25 antibody was raised against crude human synaptic immunoprecipitate.
Recognizes the human pre-synaptic protein, SNAP-25, also known as Synaptosomal-associated protein 25, Super protein (SUP) or Synaptosomal-associated 25 kD protein. SNAP-25 is a 206 amino acid Âpresynaptic protein of ~ containing two domains. Will recognize SNAP-25 fusion protein from COS cells, but not the fusion protein from bacterial systems.Âhas been used to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy.
Immunohistochemistry: LS-B3684 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3684 was determined to be 0.2-0.5 ug/ml.

Packaging

PBS, pH 7.2, 0.09% sodium azide. Sourced in TCS.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About SNAP25

P60880 NM_003081 NP_003072.2

SNAP25 Antibody, BA416N4.2 Antibody, DJ1068F16.2 Antibody, SNAP Antibody, SUP Antibody, Super protein Antibody, RIC4 Antibody, SEC9 Antibody, SNAP-25 Antibody, RIC-4 Antibody

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE.

Immunohistochemistry

Immunohistochemistry
Anti-SNAP25 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3684 concentration 0.2 ug/ml.
 

Requested From: United States
Date Requested: 12/3/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number