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Anti-SNAP25 Antibody (aa191-206) LS-C10245

Ordering

Wt. Vol. Conc. Price
- 50 µl - $485
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Rabbit Polyclonal (IgG) to Mouse SNAP25
Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Zebrafish
IHC, Western blot
Unconjugated

Details

Mouse SNAP25
Rabbit
Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
IgG Polyclonal
Antiserum

Applications

IHC, Western blot (1:1000 - 1:2000)

Specificity and Use

SNAP25 antibody was raised against a synthetic peptide from the C-terminal heptadecapeptide of mouse SNAP-25 corresponding to residues Cys+ 190-206: C-RIDEANQRATKMLGSG. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Chicken, Platypus, Lizard, Newt, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish (100%); Frog (94%); Xenopus (88%).
aa191-206
Recognizes SNAP 25 (Synaptosomal-Associated Protein). Will not detect SNAP 25 following proteolysis with botulinum neurotoxins A and E. No cross-reactivity expected with SNAP 23. Species cross-reactivity: Bovine and rat.
Suitable for use in Western Blot, Immunohistochemistry. Western Blot: 1:1000-1:2000 (using ECL). Reacts strongly with a single major band (~25kD) on denaturing Western Blots of bovine adrenal medulla and rat brain fractions. Will not detect SNAP-25 following proteolysis with botulinum neurotoxins A and E.

Packaging

0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About SNAP25

P60880 NM_003081 NP_003072.2

SNAP25 Antibody, BA416N4.2 Antibody, DJ1068F16.2 Antibody, SNAP Antibody, SUP Antibody, Super protein Antibody, RIC4 Antibody, SEC9 Antibody, SNAP-25 Antibody, RIC-4 Antibody

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE.

Requested From: United States
Date Requested: 9/24/2016

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