Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Zebrafish
(tested or 100% immunogen sequence identity)
SNAP25 antibody was raised against a synthetic peptide from the C-terminal heptadecapeptide of mouse SNAP-25 corresponding to residues Cys+ 190-206: C-RIDEANQRATKMLGSG. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Chicken, Platypus, Lizard, Newt, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish (100%); Frog (94%); Xenopus (88%).
Recognizes SNAP 25 (Synaptosomal-Associated Protein). Will not detect SNAP 25 following proteolysis with botulinum neurotoxins A and E. No cross-reactivity expected with SNAP 23. Species cross-reactivity: Bovine and rat.
Suitable for use in Western Blot, Immunohistochemistry. Western Blot: 1:1000-1:2000 (using ECL). Reacts strongly with a single major band (~25kD) on denaturing Western Blots of bovine adrenal medulla and rat brain fractions. Will not detect SNAP-25 following proteolysis with botulinum neurotoxins A and E.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.