Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Zebrafish
(tested or 100% immunogen sequence identity)
Western blot (1:1000 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
SNAP25 antibody was raised against a synthetic peptide from the C-terminal heptadecapeptide of mouse SNAP-25 corresponding to residues Cys+ 190-206: C-RIDEANQRATKMLGSG. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Chicken, Platypus, Lizard, Newt, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish (100%); Frog (94%); Xenopus (88%).
Recognizes SNAP 25 (Synaptosomal-Associated Protein). Will not detect SNAP 25 following proteolysis with botulinum neurotoxins A and E. No cross-reactivity expected with SNAP 23. Species cross-reactivity: Bovine and rat.
Suitable for use in Western Blot, Immunohistochemistry. Western Blot: 1:1000-1:2000 (using ECL). Reacts strongly with a single major band (~25kD) on denaturing Western Blots of bovine adrenal medulla and rat brain fractions. Will not detect SNAP-25 following proteolysis with botulinum neurotoxins A and E.
0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE.