SNAP25 antibody LS-C10245 is an unconjugated rabbit polyclonal antibody to SNAP25 from human, mouse, rat and other species. Validated for IHC and WB.
Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Zebrafish
(tested or 100% immunogen sequence identity)
SNAP25 antibody was raised against a synthetic peptide from the C-terminal heptadecapeptide of mouse SNAP-25 corresponding to residues Cys+ 190-206: C-RIDEANQRATKMLGSG. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Chicken, Platypus, Lizard, Newt, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish (100%); Frog (94%); Xenopus (88%).
Recognizes SNAP 25 (Synaptosomal-Associated Protein). Will not detect SNAP 25 following proteolysis with botulinum neurotoxins A and E. No cross-reactivity expected with SNAP 23. Species cross-reactivity: Bovine and rat.
Suitable for use in Western Blot, Immunohistochemistry. Western Blot: 1:1000-1:2000 (using ECL). Reacts strongly with a single major band (~25kD) on denaturing Western Blots of bovine adrenal medulla and rat brain fractions. Will not detect SNAP-25 following proteolysis with botulinum neurotoxins A and E.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.