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SNAI2 / SLUG Antibody (clone 1G7) LS‑C175177
SLUG antibody LS-C175177 is an unconjugated mouse monoclonal antibody to human SLUG (SNAI2). Validated for Flow, IF, IHC and WB.
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Review This ProductPopular SNAI2 / SLUG Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human, Mouse, Rat, Bovine, Pig, Sheep
Applications:
Western blot
Product Description
SLUG antibody LS-C175177 is an unconjugated mouse monoclonal antibody to human SLUG (SNAI2). Validated for Flow, IF, IHC and WB.
About SNAI2 / SLUG
Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells.
O43623
NM_003068
NP_003059.1
SNAI2 Antibody, SLUG Antibody, SLUGH1 Antibody, Snail homolog 2 (Drosophila) Antibody, SNAIL2 Antibody, SLUGH Antibody, Protein snail homolog 2 Antibody, WS2D Antibody, Zinc finger protein SNAI2 Antibody
Specifications
Target
Human SNAI2 / SLUG
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
[1G7]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (1:150)
- Immunofluorescence (1:100)
- Western blot (1:2000)
- Flow Cytometry (1:100)
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Immunogen
SNAI2 / SLUG antibody was raised against full length human recombinant protein of human SNAI2 (NP_003059) produced in E. coli.
Specificity
Human SLUG
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Customer Reviews (0)
Images
Immunohistochemistry
IHC of paraffin-embedded Human pancreas tissue using anti-SNAI2 mouse monoclonal antibody.
Immunofluorescence
Anti-SNAI2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAI2.
Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAI2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI2.
Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAI2 antibody, and then analyzed by flow cytometry.
Immunohistochemistry
IHC of paraffin-embedded Human pancreas tissue using anti-SNAI2 mouse monoclonal antibody.
Immunofluorescence
Anti-SNAI2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAI2.
Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAI2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI2.
Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAI2 antibody, and then analyzed by flow cytometry.
Immunohistochemistry
IHC of paraffin-embedded Human pancreas tissue using anti-SNAI2 mouse monoclonal antibody.
Immunofluorescence
Anti-SNAI2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAI2.
Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAI2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI2.
Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAI2 antibody, and then analyzed by flow cytometry.
Immunohistochemistry
IHC of paraffin-embedded Human pancreas tissue using anti-SNAI2 mouse monoclonal antibody.
Immunofluorescence
Anti-SNAI2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAI2.
Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAI2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI2.
Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAI2 antibody, and then analyzed by flow cytometry.
Requested From:
Date Requested: 6/20/2018
Date Requested: 6/20/2018
