Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
ICC (5 - 15 µg/ml)
Western blot (0.5 µg/ml)
Specificity and Use
SNAI1 / SNAIL-1 antibody was raised against e. coli-derived recombinant human Snail Pro2-Arg264 Accession # O95863.
Detects human Snail in direct ELISAs and Western blots.
Western Blot, 0.5 µg/ml, Chromatin Immunoprecipitation (ChIP), 5 ug/5 x 106 cells. Immunocytochemistry, 5-15 µg/ml, Immersion fixed SK-BR-3 human breast cancer cell line treated with MG132 (proteosome. inhibitor) and LiCl (GSK3b inhibitor).
Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
SNAI1 Antibody, DJ710H13.1 Antibody, Protein sna Antibody, SNAIL Antibody, Snail 1 Antibody, Snail 1, zinc finger protein Antibody, Snail homolog 1 (Drosophila) Antibody, SNA Antibody, Snail 1 homolog Antibody, Protein snail homolog 1 Antibody, SNAH Antibody, Snail 1 zinc finger protein Antibody, Zinc finger protein SNAI1 Antibody, SLUGH2 Antibody, SNAIL1 Antibody
Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription.
Detection of Human Snail by Western Blot. Western blot shows nuclear extracts of HeLa human cervical epithelial carcinoma cell line, A549 human lung carcinoma cell line, and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 0.5 ug/ml of Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Snail at approximately 38 kD (as indicated). This experiment was conducted under reducing conditions.
Detection of Snail-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/ml PMA and 200 ng/ml calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Snail/DNA complexes were immunoprecipitated using 5 ug Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody. immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The E-Cadherin promoter was detected by standard PCR.
Requested From: United States
Date Requested: 2/20/2017