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Anti-SNAI1 / SNAIL-1 Antibody (aa2-264) LS-C150072

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SNAI1 / SNAIL-1 Antibodies

Anti-SNAI1 / SNAIL-1 Antibody (aa80-130) IHC-plus™ LS-B7247
Rabbit Polyclonal to Human SNAI1 / SNAIL-1
Human, Mouse, Dog, Horse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SNAI1 / SNAIL-1 Antibody LS-C161335
Mouse Monoclonal (IgM,k) to Human SNAI1 / SNAIL-1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SNAI1 / SNAIL-1 Antibody (clone 2G11) LS-C198229
Mouse Monoclonal [clone 2G11] (IgG2b,k) to Human SNAI1 / SNAIL-1
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human SNAI1 / SNAIL-1
Human
ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human SNAI1 / SNAIL-1
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (0.5 µg/ml)
  • Immunoprecipitation

Specificity and Use

SNAI1 / SNAIL-1 antibody was raised against e. coli-derived recombinant human Snail Pro2-Arg264 Accession # O95863.
aa2-264
Detects human Snail in direct ELISAs and Western blots.
Western Blot, 0.5 µg/ml, Chromatin Immunoprecipitation (ChIP), 5 ug/5 x 106 cells. Immunocytochemistry, 5-15 µg/ml, Immersion fixed SK-BR-3 human breast cancer cell line treated with MG132 (proteosome. inhibitor) and LiCl (GSK3b inhibitor).

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About SNAI1 / SNAIL-1

O95863 NM_005985 NP_005976.2

SNAI1 Antibody, DJ710H13.1 Antibody, Protein sna Antibody, SNAIL Antibody, Snail 1 Antibody, Snail 1, zinc finger protein Antibody, Snail homolog 1 (Drosophila) Antibody, SNA Antibody, Snail 1 homolog Antibody, Protein snail homolog 1 Antibody, SNAH Antibody, Snail 1 zinc finger protein Antibody, Zinc finger protein SNAI1 Antibody, SLUGH2 Antibody, SNAIL1 Antibody

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription.

Western blot

Western blot
Detection of Human Snail by Western Blot. Western blot shows nuclear extracts of HeLa human cervical epithelial carcinoma cell line, A549 human lung carcinoma cell line, and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 0.5 ug/ml of Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Snail at approximately 38 kD (as indicated). This experiment was conducted under reducing conditions.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
Detection of Snail-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/ml PMA and 200 ng/ml calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Snail/DNA complexes were immunoprecipitated using 5 ug Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody. immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The E-Cadherin promoter was detected by standard PCR.

Requested From: United States
Date Requested: 12/11/2016

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