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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SMYD3 Antibody (aa7‑35) LS‑C161332 Best Seller
SMYD3 antibody LS-C161332 is an unconjugated rabbit polyclonal antibody to human SMYD3. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C161332-400
400 µl
Unavailable

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Product Description

SMYD3 antibody LS-C161332 is an unconjugated rabbit polyclonal antibody to human SMYD3. Validated for Flow, IHC and WB.
About SMYD3
Histone methyltransferase. Specifically methylates 'Lys-4' and 'Lys-5' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Q9H7B4 NM_022743 NP_073580.1

SMYD3 Antibody, BA74P14.1 Antibody, BA74P14.1 (novel protein) Antibody, KMT3E Antibody, ZMYND1 Antibody, ZNFN3A1 Antibody


Specifications

Target
Human SMYD3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8883 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C161332. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa7-35
Specificity
This SMYD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-35 amino acids from the N-terminal region of human SMYD3.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

SMYD3 Antibody IHC of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SMYD3 Antibody IHC of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of SMYD3 antibody in CEM cell line lysates (35 ug/lane). SMYD3 (arrow) was detected using the purified antibody.
Western blot of SMYD3 antibody in CEM cell line lysates (35 ug/lane). SMYD3 (arrow) was detected using the purified antibody.

Flow Cytometry

SMYD3 Antibody flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SMYD3 Antibody flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

SMYD3 Antibody IHC of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SMYD3 Antibody IHC of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of SMYD3 antibody in CEM cell line lysates (35 ug/lane). SMYD3 (arrow) was detected using the purified antibody.
Western blot of SMYD3 antibody in CEM cell line lysates (35 ug/lane). SMYD3 (arrow) was detected using the purified antibody.

Flow Cytometry

SMYD3 Antibody flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SMYD3 Antibody flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

SMYD3 Antibody IHC of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SMYD3 Antibody IHC of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of SMYD3 antibody in CEM cell line lysates (35 ug/lane). SMYD3 (arrow) was detected using the purified antibody.
Western blot of SMYD3 antibody in CEM cell line lysates (35 ug/lane). SMYD3 (arrow) was detected using the purified antibody.

Flow Cytometry

SMYD3 Antibody flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SMYD3 Antibody flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

SMYD3 Antibody IHC of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SMYD3 Antibody IHC of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of SMYD3 antibody in CEM cell line lysates (35 ug/lane). SMYD3 (arrow) was detected using the purified antibody.
Western blot of SMYD3 antibody in CEM cell line lysates (35 ug/lane). SMYD3 (arrow) was detected using the purified antibody.

Flow Cytometry

SMYD3 Antibody flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SMYD3 Antibody flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/25/2018

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