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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SMS / Spermine Synthase Antibody (clone 5F9) LS‑C173074
Spermine Synthase antibody LS-C173074 is an unconjugated mouse monoclonal antibody to human Spermine Synthase (SMS). Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C173074-100
100 µl (0.36 mg/ml)
Unavailable

Popular SMS / Spermine Synthase Products

SMS Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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IHC of paraffin-embedded Human prostate tissue using anti-SMS mouse monoclonal antibody.
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Product Description

Spermine Synthase antibody LS-C173074 is an unconjugated mouse monoclonal antibody to human Spermine Synthase (SMS). Validated for Flow, IF and WB.
About SMS / Spermine Synthase
SMS / Spermine Synthase is a protein belonging to the spermidine/spermin synthase family. Pseudogenes of this gene are located on chromosomes 1, 5, 6 and X. Mutations in this gene are associated with X-linked Snyder-Robinson mental retardation syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. P52788 NM_004595 NP_004586.2

SMS Antibody, MRSR Antibody, Spermine synthase Antibody, SPMSY Antibody, SPS Antibody, SRS Antibody


Specifications

Target
Human SMS / Spermine Synthase
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [5F9]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)
Immunogen
Full length human recombinant protein of human SMS (NP_004586) produced in HEK293T cell.
Specificity
Human Spermine Synthase / SMS
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Anti-SMS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS.
Anti-SMS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-SMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot of extracts (35 ug) from 9 different cell lines by using anti-SMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunofluorescence

Anti-SMS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS.
Anti-SMS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-SMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot of extracts (35 ug) from 9 different cell lines by using anti-SMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunofluorescence

Anti-SMS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS.
Anti-SMS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-SMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot of extracts (35 ug) from 9 different cell lines by using anti-SMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunofluorescence

Anti-SMS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS.
Anti-SMS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-SMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot of extracts (35 ug) from 9 different cell lines by using anti-SMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-SMS antibody (Red), compared to a nonspecific negative control antibody (Blue).


Requested From: 
Date Requested: 6/18/2018

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