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Anti-SMG1 / ATX Antibody (clone 1C12) LS-C133700

Catalog Size Price
LS-C133700-100 100 µg Unavailable

Related Products

26 SMG1 / ATX Antibodies

Most Popular SMG1 / ATX Antibodies

Anti-SMG1 / ATX Antibody (clone 1C12) LS-C133700
Mouse Monoclonal [clone 1C12] (IgG1,k) to Human SMG1 / ATX
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml]
Anti-SMG1 / ATX Antibody (aa2925-2975) LS-C287285
Rabbit Polyclonal (IgG) to Human SMG1 / ATX
Human, Mouse, Bovine, Dog, Rabbit, Chicken
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Detection of Human SMG1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMG1 used at a dilution of 1:500 (2 ug/ml). Detection: DAB.
Anti-SMG1 / ATX Antibody (C-Terminus) LS-C328691
Rabbit Polyclonal (IgG) to Human SMG1 / ATX
Human
IHC, ELISA
Unconjugated
Anti-SMG1 / ATX Antibody (Internal) LS-C328693
Rabbit Polyclonal (IgG) to Human SMG1 / ATX
Human
IHC, ELISA
Unconjugated
Anti-SMG1 / ATX Antibody (N-Terminus) LS-C330659
Rabbit Polyclonal (IgG) to Human SMG1 / ATX
Human
IHC, ELISA
Unconjugated

100% Guaranteed
Mouse Monoclonal [clone 1C12] (IgG1,k) to Human SMG1 / ATX
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SMG1 / ATX
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1C12]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1.5 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

SMG1 / ATX antibody was raised against sMG1 (-, 2922 a.a. ~ 3032 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human Smg1
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About SMG1 / ATX

Q96Q15 NM_015092 NP_055907.3

SMG1 Antibody, HSMG-1 Antibody, KIAA0421 Antibody, Lambda-interacting protein Antibody, LIP Antibody, SMG-1 Antibody

Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml]
Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml]

Western blot

SMG1 monoclonal antibody (M03), clone 1C12 Western blot of SMG1 expression in HeLa NE.
SMG1 monoclonal antibody (M03), clone 1C12 Western blot of SMG1 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SMG1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SMG1 is approximately 0.1 ng/ml as a capture antibody.

Requested From: 
Date Requested: 4/21/2018

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