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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SMG1 / ATX Antibody (clone 1C12) LS‑C133700 Best Seller
ATX antibody LS-C133700 is an unconjugated mouse monoclonal antibody to human ATX (SMG1). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C133700-100
100 µg
Unavailable

Popular SMG1 / ATX Products

Detection of Human SMG1 by Immunohistochemistry. Sample: FFPE section of human breast adenocarcinoma Antibody: Affinity purified rabbit anti-SMG1 used at a dilution of 1:250. Detection: DAB.
Species: Human, Mouse, Bovine, Dog, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Immunofluorescence
Detection of Human SMG1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMG1 used at a dilution of 1:500 (2 ug/ml). Detection: DAB.
Species: Human, Mouse, Bovine, Dog, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: IHC, ELISA
Antibody
Species: Human
Applications: IHC, ELISA

Product Description

ATX antibody LS-C133700 is an unconjugated mouse monoclonal antibody to human ATX (SMG1). Validated for ELISA, IHC and WB.
About SMG1 / ATX
Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Q96Q15 NM_015092 NP_055907.3

SMG1 Antibody, HSMG-1 Antibody, KIAA0421 Antibody, Lambda-interacting protein Antibody, LIP Antibody, SMG-1 Antibody


Specifications

Target
Human SMG1 / ATX
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1C12]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1.5 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SMG1 / ATX antibody was raised against sMG1 (-, 2922 a.a. ~ 3032 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human Smg1
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml]
Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml]

Western blot

SMG1 monoclonal antibody (M03), clone 1C12 Western blot of SMG1 expression in HeLa NE.
SMG1 monoclonal antibody (M03), clone 1C12 Western blot of SMG1 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SMG1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SMG1 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml]
Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml]

Western blot

SMG1 monoclonal antibody (M03), clone 1C12 Western blot of SMG1 expression in HeLa NE.
SMG1 monoclonal antibody (M03), clone 1C12 Western blot of SMG1 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SMG1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SMG1 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml]
Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml]

Western blot

SMG1 monoclonal antibody (M03), clone 1C12 Western blot of SMG1 expression in HeLa NE.
SMG1 monoclonal antibody (M03), clone 1C12 Western blot of SMG1 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SMG1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SMG1 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml]
Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml]

Western blot

SMG1 monoclonal antibody (M03), clone 1C12 Western blot of SMG1 expression in HeLa NE.
SMG1 monoclonal antibody (M03), clone 1C12 Western blot of SMG1 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SMG1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SMG1 is approximately 0.1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/19/2018

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