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Anti-SMC4 Antibody (aa281-297) IHC-plus™ LS-B2066

Note: This antibody replaces LS-C656, LS-C77678
Catalog Size Price
LS-B2066-50 50 µg (0.5 mg/ml) Unavailable

Related Products

26 SMC4 Antibodies

Most Popular SMC4 Antibodies

Anti-SMC4 Antibody (aa281-297) IHC-plus™ LS-B2066
Rabbit Polyclonal (IgG) to Human SMC4
Human
IHC - Paraffin, Western blot
Unconjugated
Anti-SMC4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2066 concentration 5 ug/ml.
Anti-SMC4 Antibody (aa47-61) IHC-plus™ LS-B2067
Rabbit Polyclonal (IgG) to Human SMC4
Human
IHC - Paraffin, Western blot
Unconjugated
Anti-SMC4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2067 concentration 5 ug/ml.
Anti-SMC4 Antibody (aa600-650) LS-C287004
Rabbit Polyclonal (IgG) to Human SMC4
Human, Mouse, Rat, Dog, Guinea pig, Horse, Rabbit
ICC, Immunofluorescence, Western blot
Unconjugated
Localization of SMC4 to Mitotic Chromosomes by Immunofluorescence. Samples: HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody: Affinity purified rabbit anti-SMC4 antibody used at 5 ug/ml. Detection: Rhodamine labeled anti-rabbit secondary antibody. Cells were counterstained with DAPI.
Anti-SMC4 Antibody (C-Terminus) LS-C287005
Rabbit Polyclonal (IgG) to Human SMC4
Human, Rat, Bovine, Guinea pig, Pig, Rabbit
ICC, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Unconjugated
Detection of Human SMC4 by Western Blot and Immuno-precipitation. Samples: Whole cell lysate (90 ug for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Antibodies: Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 ug/ml for WB and 5 ug for IP. Detection: Chemiluminescence with the indicated exposure times.
Anti-SMC4 Antibody LS-C403587
Rabbit Polyclonal (IgG) to Human SMC4
Human, Mouse
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry of Human esophagus cancer using SMC4 Polyclonal Antibody at dilution of 1:35.

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Rabbit Polyclonal (IgG) to Human SMC4
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human SMC4
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)

Specificity and Use

SMC4 antibody was raised against synthetic peptide from human SMC4.
aa281-297
A synthetic peptide corresponding to amino acids 281-297 of human CAP-C. Genbank accession no. NP_001002800.
Immunohistochemistry: LS-B2066 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2066 was determined to be 5 ug/ml.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About SMC4

Q9NTJ3 NM_005496 NP_005487.3

SMC4 Antibody, HCAP-C Antibody, SMC protein 4 Antibody, SMC4L1 Antibody, SMC-4 Antibody, XCAP-C homolog Antibody, CAP-C Antibody, CAPC Antibody

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

Immunohistochemistry

Anti-SMC4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2066 concentration 5 ug/ml.
Anti-SMC4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2066 concentration 5 ug/ml.

Western blot

Western blot of CAP-C in Ramos cell lysate using antibody at 2 ug/ml.
Western blot of CAP-C in Ramos cell lysate using antibody at 2 ug/ml.

Requested From: 
Date Requested: 4/20/2018

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