Human SMC1A / SMC1

Mouse

Human, Monkey, Bovine, Dog, Rabbit (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

KLH-conjugated, synthetic peptide corresponding to aa 951-962, (SQEEGS[pS]QGEDS) of human SMC1 (Structural Maintenance of Chromosome 1). The immunizing sequence is identical in bovine and has 11/12 identical amino acids in mouse and xenopus.

pSer957

Recognizes phospho-SMC1 (Ser957), Mr 150kD. Species cross-reactivity: Human and mouse. Predicted to cross-react with Xenopus and bovine based on sequence homology.

• Western blot (1 - 2 µg/ml)

Suitable for use in Western Blot. Western Blot: 1-2 ug/ml detects phospho-SMC1 in RIPA lysates from irradiated GMO536 cells and hydroxyurea treated HEK293 cells. Cell lysates from untreated and irradiated GMO536 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-SMC1 (Ser957) (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q14683 NCBI: NM_006306 NP_006297.2