Human SMC1A / SMC1
Human, Monkey, Bovine, Dog, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (1 - 2 µg/ml)
- (applications tested for the base form of this product only)
SMC1A / SMC1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 951-962 SQEEGS[pS]QGEDS) of human SMC1 (Structural Maintenance of Chromosome 1). The immunizing sequence is identical in bovine and has 11/12 identical amino acids in mouse and xenopus.
Recognizes phospho-SMC1 (Ser957), Mr 150kD. Species cross-reactivity: Human and mouse. Predicted to cross-react with Xenopus and bovine based on sequence homology.
Suitable for use in Western Blot. Western Blot: 1-2 ug/ml detects phospho-SMC1 in RIPA lysates from irradiated GMO536 cells and hydroxyurea treated HEK293 cells. Cell lysates from untreated and irradiated GMO536 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-SMC1 (Ser957) (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
For research use only.
This antibody carries the LSBio 100% Guarantee.