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Anti-SMARCE1 / BAF57 Antibody (aa364-376) IHC-plus™ LS-B2752

Note: This antibody replaces LS-C54564, LS-C74595

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SMARCE1 / BAF57 Antibodies

Anti-SMARCE1 / BAF57 Antibody (aa364-376) IHC-plus™ LS-B2752
Goat Polyclonal to Human SMARCE1 / BAF57
Human, Monkey, Bat, Horse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SMARCE1 / BAF57 Antibody (clone 6G11) LS-C198225
Mouse Monoclonal [clone 6G11] (IgG1,k) to Human SMARCE1 / BAF57
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunoprecipitation Image
Anti-SMARCE1 / BAF57 Antibody (N-Terminus) IHC-plus™ LS-B11494
Rabbit Polyclonal (IgG) to Human SMARCE1 / BAF57
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human SMARCE1 / BAF57
Human, Monkey, Bat, Horse
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SMARCE1 / BAF57
Goat
Human, Monkey, Bat, Horse (tested or 100% immunogen sequence identity)
Mouse, Rat, Dog (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.2 - 0.6 µg/ml)
  • ELISA (1:16000)

Specificity and Use

SMARCE1 / BAF57 antibody was raised against synthetic peptide C-PPTDPIPEDEKKE from the C-terminus of human SMARCE1 (NP_003070.3). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Bat, Horse (100%); Mouse, Rat, Elephant, Panda, Dog (92%).
LS-E25584 - Lyophilized - 100 µg - $145.00
aa364-376
Human SMARCE1.
Immunohistochemistry: LS-B2752 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2752 was determined to be 3.75 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About SMARCE1 / BAF57

Q969G3 NM_003079 NP_003070.3

SMARCE1 Antibody, BRG1-associated factor 57 Antibody, BAF57 Antibody

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state.

Immunohistochemistry

Immunohistochemistry
Anti-SMARCE1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2752 concentration 75 ug/ml.

Western blot

Western blot
HEK293 overexpressing BAF57 (RC209444) and probed with the antibody (mock transfection in first lane).

Western blot

Western blot
Antibody staining (0.2 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
 

Requested From: United States
Date Requested: 12/8/2016

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