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Anti-SMARCAL1 Antibody (aa80-96) IHC-plus™ LS-B423

Note: This antibody replaces LS-C19028, LS-C67424

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SMARCAL1 Antibodies

Anti-SMARCAL1 Antibody (aa80-96) IHC-plus™ LS-B423
Rabbit Polyclonal to Human SMARCAL1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SMARCAL1 Antibody LS-C332054
Rabbit Polyclonal (IgG) to Human SMARCAL1
Human, Mouse, Rat
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SMARCAL1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SMARCAL1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:3000)
  • ELISA (1:16000 - 1:64000)

Specificity and Use

SMARCAL1 antibody was raised against synthetic peptide from human SMARCAL1.
aa80-96
aa 80-96 of human SmarcAL1 protein.
Immunohistochemistry: LS-B423 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B423 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About SMARCAL1

Q9NZC9 NM_014140 NP_054859.2

SMARCAL1 Antibody, ATP-driven annealing helicase Antibody, HARP Antibody, HepA-related protein Antibody, HHARP Antibody, SMARCA-like protein 1 Antibody, SIOD Antibody

ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks.

Immunohistochemistry

Immunohistochemistry
Anti-SMARCAL1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B423 concentration 5 ug/ml.

Western blot

Western blot
Anti-SmarcAL1 Antibody - Western Blot. Western blot of Affinity Purified anti-SmarcAL1 antibody shows detection of a band ~106 kD band (arrowhead) corresponding to SmarcAL1 in human derived cultured cell lysates (HeLa nuclear extract lane 1, HeLa lane 2, A431 lane 3, Jurkat lane 4, and 293 lane 5). Approximately 5 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SmarcAL1 antibody. Signal was detected using standard techniques. SmarcAL1 is the band seen between the 97 and 116kD markers.

Requested From: United States
Date Requested: 12/10/2016

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