Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone BRG-01] (IgG2a) to Human SMARCA4 / BRG1
IHC - Paraffin, Western blot
Human SMARCA4 / BRG1
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [BRG-01]
Protein A purified
IHC - Paraffin (10 µg/ml)
Western blot (10 µg/ml)
Specificity and Use
SMARCA4 / BRG1 antibody was raised against bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.
The antibody BRG-01 recognizes an epitope within C-terminal part of human Brg1, a 205 kD catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.
Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody,
PBS, 15 mM sodium azide, pH 7.4. Sourced in Ascites.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex.