Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:100)
Western blot (1:2000 - 1:10000)
Specificity and Use
SMARCA4 / BRG1 antibody was raised against synthetic peptide corresponding to amino acid residues 75 and 125 of human BRM/SWI2-Related Gene 1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of chromatin, Subfamily A, member 4) using the numbering given in entry NP_003063.2 (GeneID 6597).
Species cross-reactivity: Human and mouse. Other species have not been tested.
Suitable for use in Western Blot, Immunohistochemistry with FFPE tissues and Immunoprecipitation. Immunoprecipitation: 2-5 ug/mg lysate. Western Blot: 1:2000-1:10000.
TBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
Transcription factor Foxp3 and its protein partners form a complex regulatory network. Rudra D, deRoos P, Chaudhry A, Niec RE, Arvey A, Samstein RM, Leslie C, Shaffer SA, Goodlett DR, Rudensky AY. Nature immunology. 2012 Oct;13:1010-9. [Full Text Article]
Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex.