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Anti-SMAD7 Antibody (aa36-50) IHC-plus™ LS-B120

Note: This antibody replaces LS-C807

Ordering

Wt. Vol. Conc. Price
50 µg 100 µl 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SMAD7 Antibodies

Anti-SMAD7 Antibody (aa36-50) IHC-plus™ LS-B120
Rabbit Polyclonal (IgG) to Human SMAD7
Human, Monkey, Bat, Bovine, Dog
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SMAD7 Antibody (clone 293039) LS-C150142
Mouse Monoclonal [clone 293039] (IgG2b) to Human SMAD7
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SMAD7 Antibody (aa375-389) LS-C313088
Rabbit Polyclonal (IgG) to Human SMAD7
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human SMAD7
Human, Monkey, Bat, Bovine, Dog
IHC - Paraffin, Western blot
Unconjugated

Details

Human SMAD7
Rabbit
Human, Monkey, Bat, Bovine, Dog (tested or 100% immunogen sequence identity)
Mouse, Rat, Pig, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:500 - 1:1000)

Specificity and Use

SMAD7 antibody was raised against amino acids 12-29 (RLWRSRAPGGEDEEEGAG) and 36-50 (ELRGEGATDSRAHGA) of human SMAD7. This sequence is 100% or highly conserved between human and multiple species. Percent identity by BLAST analysis: Human, Monkey, Bovine, Dog, Bat, Panda (100%); Mouse, Rat, Rabbit, Pig, Platypus (94%).
aa36-50
Two synthetic peptides corresponding to aa 12-18 (RLWRSRAPGGEDEEEGAG) and amino acids 36-50 (ELRGEGATDSRAHGA) of human SMAD7; GenBank Accession no. AAB81354.1. This sequence is 100% or highly conserved between human and multiple species.
Immunohistochemistry: LS-B120 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B120 was determined to be 10 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SMAD7

O15105 NM_005904 NP_005895.1

SMAD7 Antibody, CRCS3 Antibody, HSMAD7 Antibody, MAD homolog 7 Antibody, SMAD 7 Antibody, MADH7 Antibody, MADH8 Antibody, Mothers against DPP homolog 7 Antibody, Mothers against DPP homolog 8 Antibody, MAD homolog 8 Antibody, SMAD family member 7 Antibody

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation.

Immunohistochemistry

Immunohistochemistry
Anti-SMAD7 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B120 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/5/2016

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