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Anti-SMAD3 Antibody (phospho-Ser423/Ser425) IHC-plus™ LS-B64

Note: This antibody replaces LS-C18973, LS-C56911


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

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Rabbit Polyclonal to Human SMAD3
Human, Mouse, Rat, Bovine, Pig, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA


Human SMAD3
Human, Mouse, Rat, Bovine, Pig, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Immunoaffinity purified


IHC - Paraffin (2.5 µg/ml), Immunofluorescence, Western blot (1:2000 - 1:20000), Flow Cytometry, ELISA (1:75000 - 1:300000)

Specificity and Use

SMAD3 antibody was raised against synthetic peptide from human Smad3.
Amino acids 417-425 of human SMAD3 protein.
Immunohistochemistry: LS-B64 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B64 was determined to be 2.5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About SMAD3

P84022 NM_005902 NP_005893.1

SMAD3 Antibody, HSPC193 Antibody, HMAD-3 Antibody, HsT17436 Antibody, LDS1C Antibody, LDS3 Antibody, Mad homolog JV15-2 Antibody, MAD3 Antibody, MADH3 Antibody, Mothers against DPP homolog 3 Antibody, JV15-2 Antibody, SMA- and MAD-related protein 3 Antibody, SMAD family member 3 Antibody, MAD homolog 3 Antibody, HSMAD3 Antibody, Mad protein homolog Antibody, SMAD 3 Antibody

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription.


Anti-SMAD3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B64 concentration 5 ug/ml.

Requested From: United States
Date Requested: 11/26/2015

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