Human SMAD3

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Mouse, Rat (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein.

A BLAST analysis was used to suggest cross-reactivity with Smad3 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. Also, the antibody is Smad3 specific, and reactivity to other Smad proteins (specifically Smad1, Smad2, Smad4, and Smad7) is not detected in over-expressed cell lysates (Personal Communication, Kathleen Flanders, CCR-NCI, Bethesda, MD).

• Western blot (1:500 - 1:10000)
• ELISA (1:15000 - 1:50000)

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kD in size corresponding to Smad3 protein by western blotting in the appropriate cell lysate or extract.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: -20°C; Long term: -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P84022 NCBI: NM_005902 NP_005893.1