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Anti-SMAD3 Antibody (clone AF9F7) IHC-plus™ LS-B2555

Note: This antibody replaces LS-C62161


Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular SMAD3 Antibodies

Anti-SMAD3 Antibody (phospho-Ser423/Ser425) IHC-plus™ LS-B64
Rabbit Polyclonal to Human SMAD3
Human, Mouse, Rat, Bovine, Pig, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Immunohistochemistry Image
Anti-SMAD3 Antibody (C-Terminus) LS-C46894
Rabbit Polyclonal to Human SMAD3
Western blot, ELISA
Anti-SMAD3 Antibody (aa100-150) IHC-plus™ LS-B2064
Rabbit Polyclonal to Human SMAD3
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Macaque
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone AF9F7] (IgG1,k) to Human SMAD3
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA


Human SMAD3
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [AF9F7]


  • IHC - Paraffin (1:50)
  • Western blot (1:1000)
  • Immunoprecipitation

Specificity and Use

SMAD3 antibody was raised against recombinant human protein purified from E. coli (His-Smad3).
Immunohistochemistry: LS-B2555 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2555 was determined to be 1:50.


HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About SMAD3

P84022 NM_005902 NP_005893.1

SMAD3 Antibody, HSPC193 Antibody, HMAD-3 Antibody, HsT17436 Antibody, LDS1C Antibody, LDS3 Antibody, Mad homolog JV15-2 Antibody, MAD3 Antibody, MADH3 Antibody, Mothers against DPP homolog 3 Antibody, JV15-2 Antibody, SMA- and MAD-related protein 3 Antibody, SMAD family member 3 Antibody, MAD homolog 3 Antibody, HSMAD3 Antibody, Mad protein homolog Antibody, SMAD 3 Antibody

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription.


Anti-SMAD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2555 dilution 1:50.

Western blot

Western blot

Requested From: United States
Date Requested: 10/24/2016

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