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Anti-SMAD3 Antibody (clone 7F3) IHC-plus™ LS-B5459

Note: This antibody replaces LS-C133306

Ordering

Wt. Vol. Conc. Price
50 µg - 0.43 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SMAD3 Antibodies

Anti-SMAD3 Antibody (aa100-150) IHC-plus™ LS-B2064
Rabbit Polyclonal to Human SMAD3
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Macaque
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SMAD3 Antibody (C-Terminus) LS-C46894
Rabbit Polyclonal to Human SMAD3
Human
Western blot, ELISA
Unconjugated
Anti-SMAD3 Antibody (phospho-Ser423/Ser425) IHC-plus™ LS-B64
Rabbit Polyclonal to Human SMAD3
Human, Mouse, Rat, Bovine, Pig, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 7F3] (IgG1,k) to Human SMAD3
Human, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SMAD3
Mouse
Human, Rat (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [7F3]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

SMAD3 antibody was raised against sMAD3 (NP_005893, 120 a.a. ~ 222 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human SMAD3
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western blot using tissue lysates, cell lysates and the recombinant protein used as the immunogen.

Packaging

PBS, pH 7.2
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About SMAD3

P84022 NM_005902 NP_005893.1

SMAD3 Antibody, HSPC193 Antibody, HMAD-3 Antibody, HsT17436 Antibody, LDS1C Antibody, LDS3 Antibody, Mad homolog JV15-2 Antibody, MAD3 Antibody, MADH3 Antibody, Mothers against DPP homolog 3 Antibody, JV15-2 Antibody, SMA- and MAD-related protein 3 Antibody, SMAD family member 3 Antibody, MAD homolog 3 Antibody, HSMAD3 Antibody, Mad protein homolog Antibody, SMAD 3 Antibody

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription.

Immunohistochemistry

Immunohistochemistry
Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5459 concentration 2.5 ug/ml.

Western blot

Western blot
SMAD3 monoclonal antibody clone 7F3. Western blot of SMAD3 expression in human colon.

Western blot

Western blot
SMAD3 monoclonal antibody clone 7F3. Western blot of SMAD3 expression in Jurkat.

Western blot

Western blot
SMAD3 monoclonal antibody clone 7F3. Western blot of SMAD3 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged SMAD3 is approximately 0.1 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/8/2016

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