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Anti-SMAD3 Antibody (aa127-156) LS-C161296

Catalog Size Price
LS-C161296-400 400 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human SMAD3
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human SMAD3
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Pig, Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

LS-E2505 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C161296. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa127-156
This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from the Central region of human SMAD3.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About SMAD3

P84022 NM_005902 NP_005893.1

SMAD3 Antibody, HSPC193 Antibody, HMAD-3 Antibody, HsT17436 Antibody, LDS1C Antibody, LDS3 Antibody, Mad homolog JV15-2 Antibody, MAD3 Antibody, MADH3 Antibody, Mothers against DPP homolog 3 Antibody, JV15-2 Antibody, SMA- and MAD-related protein 3 Antibody, SMAD family member 3 Antibody, MAD homolog 3 Antibody, HSMAD3 Antibody, Mad protein homolog Antibody, SMAD 3 Antibody

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription.

Immunohistochemistry

SMAD3 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human heart tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SMAD3 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human heart tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of SMAD3 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of SMAD3 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

SMAD3 Antibody western blot of CEM cell line lysates (35 ug/lane). The SMAD3 antibody detected the SMAD3 protein (arrow).
SMAD3 Antibody western blot of CEM cell line lysates (35 ug/lane). The SMAD3 antibody detected the SMAD3 protein (arrow).

Requested From: 
Date Requested: 5/25/2018

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