Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Western blot (0.1 - 1 µg/ml)
Specificity and Use
SMAD2 antibody was raised against synthetic peptide encompassing the phosphorylated serine 465 and 467 residues of human, mouse and rat Smad2.
Detects the ~58kD Smad2 when dually phosphorylated at Ser465 and Ser467. While TGF-beta-stimulated HEPG2 lysates yielded the target band in Western Blot, the unstimulated HEPG2 lysates did not show a signal. Reactivity has been confirmed by Western Blot with TGF-beta-stimulated HEPG2 human hepatocellular carcinoma lysates.
Suitable for use in Western Blot. Western Blot: 0.1-1 ug/ml.
PBS, pH 7.4, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
SMAD2 Antibody, HMAD-2 Antibody, HSMAD2 Antibody, JV18-1 Antibody, Mad protein homolog smad2 Antibody, MADR2 Antibody, MADH2 Antibody, SMAD 2 Antibody, MAD homolog 2 Antibody, Mad-related protein 2 Antibody, JV18 Antibody, Mother against DPP homolog 2 Antibody, Mothers against DPP homolog 2 Antibody, Sma- and Mad-related protein 2 Antibody, SMAD family member 2 Antibody
SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.