(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Western blot (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
SMAD2 antibody was raised against synthetic peptide encompassing the phosphorylated serine 465 and 467 residues of human, mouse and rat Smad2.
Detects the ~58kD Smad2 when dually phosphorylated at Ser465 and Ser467. While TGF-beta-stimulated HEPG2 lysates yielded the target band in Western Blot, the unstimulated HEPG2 lysates did not show a signal. Reactivity has been confirmed by Western Blot with TGF-beta-stimulated HEPG2 human hepatocellular carcinoma lysates.
Suitable for use in Western Blot. Western Blot: 0.1-1 ug/ml.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.