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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SMAD2 Antibody (MH1 Domain) LS‑C10216
Antibody
SMAD2 antibody LS-C10216 is an unconjugated rabbit polyclonal antibody to human SMAD2. Validated for ELISA and WB.
Catalog
Size
Price
LS-C10216-100
100 µg
Unavailable

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Product Description

SMAD2 antibody LS-C10216 is an unconjugated rabbit polyclonal antibody to human SMAD2. Validated for ELISA and WB.
About SMAD2
SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. Q15796 NM_005901 NP_005892.1

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Specifications

Target
Human SMAD2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
SMAD2 antibody was raised against a 27 amino acid synthetic peptide derived from the MH1 domain of human Smad2. Species sequence Homology: Mouse (24/27aa); Xenopus (26/27aa).
Epitope
MH1 Domain
Specificity
Recognizes human Smad2 and can be used to detect the predominant splice form of Smad2. Cross-reactivity with Smad3 or with other Smad family members has not been observed. Antibody reactivity was confirmed by Western Blot using lysates derived from Smad2-transfected COS cells. Species sequence Homology: Mouse (24/27aa); Xenopus (26/27aa).
Usage
Suitable for use in ELISA and Western Blot. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml.
Presentation
PBS, pH 7.4, 0.05% sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/22/2018

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