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Anti-SMAD2 Antibody (Internal) LS-C60127

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SMAD2 Antibodies

Anti-SMAD2 Antibody (aa234-249) IHC-plus™ LS-B2063
Rabbit Polyclonal to Human SMAD2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SMAD2 Antibody (clone 3G6) IHC-plus™ LS-B5454
Mouse Monoclonal [clone 3G6] (IgG2b,k) to Human SMAD2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SMAD2
Human, Mouse, Rat
Western blot, ELISA
Unconjugated

Details

Human SMAD2
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:1000 - 1:3000)
  • ELISA (1:100000)

Specificity and Use

SMAD2 antibody was raised against synthetic peptide corresponding to an internal region of human Smad23 protein.
Internal
human Smad2 protein. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologs from other sources is not known. Also
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kD in size corresponding to Smad2 protein by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About SMAD2

Q15796 NM_005901 NP_005892.1

SMAD2 Antibody, HMAD-2 Antibody, HSMAD2 Antibody, JV18-1 Antibody, Mad protein homolog smad2 Antibody, MADR2 Antibody, MADH2 Antibody, SMAD 2 Antibody, MAD homolog 2 Antibody, Mad-related protein 2 Antibody, JV18 Antibody, Mother against DPP homolog 2 Antibody, Mothers against DPP homolog 2 Antibody, Sma- and Mad-related protein 2 Antibody, SMAD family member 2 Antibody

SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.

Western blot

Western blot
Anti-Smad2 Antibody - Western Blot. Western blot of affinity purified anti-Smad2 to detect over-expressed Smad2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of Smad2 in lysates of COS transfected with Smad2. Lane V contains lysates of MDA-MB231 cells treated with vehicle; the next lane contains lysates of MDA-MB231 cells treated with TGF?. Low levels of staining in control lanes correspond to detection of endogenous Smad2. Preincubation of the antibody with immunizing peptide (data not shown) completely blocks specific band staining. The blot presented is askew relative to the molecular weight markers. The expected MW for Smad2 is 52 kD. The membrane was probed with the primary antibody at a 1:2500 dilution. Personal Communication Kathleen Flanders, CCR-NCI, Bethesda, MD.
 

Requested From: United States
Date Requested: 2/21/2017

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