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| Catalog Number | Size | Price |
|---|---|---|
| LS-C39124-100 | 100 µg | $832 |
Polyclonal Rabbit anti‑Human SMAD1 Antibody (phospho‑Ser463/Ser465, WB) LS‑C39124
Polyclonal Rabbit anti‑Human SMAD1 Antibody (phospho‑Ser463/Ser465, WB) LS‑C39124
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
SMAD1 antibody LS-C39124 is an unconjugated rabbit polyclonal antibody to SMAD1 (pSer463/Ser465) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
Target
Human SMAD1
Synonyms
SMAD1 | BSP1 | BSP-1 | JV4-1 | MADH1 | Mothers against DPP homolog 1 | JV41 | SMAD family member 1 | TGF-beta signaling protein 1 | MADR1 | HSMAD1 | MAD homolog 1 | Mad-related protein 1 | SMAD 1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Xenopus
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Synthetic peptide (CG-GSPHNPIS[pS]V[pS]-GG) corresponding to amino acids 455-465 of human Smad1 protein (KLH).
Epitope
pSer463/Ser465
Specificity
Recognizes the dual serine phosphorylated human Smad1 (Ser 463/465), which in the phosphorylated state has an apparent molecular weight of approximately 60-65kD. Species cross-reactivity: Mouse (Predicted cross-reactivity with human, rat and Xenopus).
Applications
- Western blot (0.5 - 2 µg/ml)
Usage
Suitable for use in Western Blot. Western Blot: 0.5-2 ug/ml detected phosphorylated Smad1 in RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4. P19 cell lysate treated with BMP4 was resolved by electrophoresis, transferred to nitrocellulose and probed with phospho-Smad1 (Ser463/465) (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Note: A non-specific band was detected in P19 cell lysate, Mr 34-35kD.
Presentation
0.1 M Tris-glycine, pH 7.2, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SMAD1
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Featured Products
Species:
Human, Mouse, Rat
Applications:
ICC, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat, Dog
Applications:
Western blot, Immunoprecipitation, ELISA
![SMAD1 Antibody - Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/smad1-antibody-clone-1d3-ls-c133298/51116_1275040.jpg)
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Sheep, Chicken
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
