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Anti-SMAD1 Antibody (clone 1D3) LS-C133298

Catalog Size Price
LS-C133298-100 100 µg (0.5 mg/ml) Unavailable

Related Products

120 SMAD1 Antibodies

Most Popular SMAD1 Antibodies

Anti-SMAD1 Antibody (C-Terminus) LS-C31108
Rabbit Polyclonal (IgG) to Human SMAD1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SMAD1 Antibody LS-C75853
Mouse Monoclonal (IgG1) to Human SMAD1
Human, Mouse, Rat
ICC, Western blot, Immunoprecipitation, Flow Cytometry
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Anti-SMAD1 Antibody (N-Terminus) LS-C80224
Rabbit Polyclonal (IgG) to Human SMAD1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila, Insect
IHC - Paraffin, Western blot
Unconjugated
SMAD1 antibody LS-C80224 was used in IHC to stain formalin-fixed, paraffin-embedded human skin. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SMAD1 Antibody (clone 1D3) LS-C133298
Mouse Monoclonal [clone 1D3] (IgG1,k) to Human SMAD1
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
Anti-SMAD1 Antibody (phospho-Ser206) LS-C154249
Rabbit Polyclonal (IgG) to Human SMAD1
Human, Mouse, Rat, Dog
Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-SMAD1 Antibody - Western Blot. Western blot of Affinity Purified anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37?C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (B501-0500) and reacted for 1 hour at room temperature. Anti-beta actin (p/n LS-B323) staining was used as a loading control. The membrane was washed and reacted with a 1:3000 dilution HRP-conjugated a-Rabbit IgG (p/n LS-C60865) for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.

100% Guaranteed
Mouse Monoclonal [clone 1D3] (IgG1,k) to Human SMAD1
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml] Western blot of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody (M02), clone 1D3.
SMAD1 monoclonal antibody (M02), clone 1D3. Western blot of SMAD1 expression in MES-SA/Dx5. SMAD1 monoclonal antibody (M02), clone 1D3. Western blot of SMAD1 expression in A-549.
SMAD1 monoclonal antibody (M02), clone 1D3 Western blot of SMAD1 expression in IMR-32. Detection limit for recombinant GST tagged SMAD1 is approximately 3 ng/ml as a capture antibody.

Details

Human SMAD1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1D3]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

  • IHC - Paraffin

Specificity and Use

SMAD1 antibody was raised against sMAD1 (NP_005891, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human SMAD1
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About SMAD1

Q15797 NM_005900 NP_005891.1

SMAD1 Antibody, BSP1 Antibody, BSP-1 Antibody, JV4-1 Antibody, MADH1 Antibody, Mothers against DPP homolog 1 Antibody, JV41 Antibody, SMAD family member 1 Antibody, TGF-beta signaling protein 1 Antibody, MADR1 Antibody, HSMAD1 Antibody, MAD homolog 1 Antibody, Mad-related protein 1 Antibody, SMAD 1 Antibody

SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses.
(More About SMAD1)

Immunofluorescence

Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody (M02), clone 1D3.
Western blot of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody (M02), clone 1D3.

Western blot

SMAD1 monoclonal antibody (M02), clone 1D3. Western blot of SMAD1 expression in MES-SA/Dx5.
SMAD1 monoclonal antibody (M02), clone 1D3. Western blot of SMAD1 expression in MES-SA/Dx5.

Western blot

SMAD1 monoclonal antibody (M02), clone 1D3. Western blot of SMAD1 expression in A-549.
SMAD1 monoclonal antibody (M02), clone 1D3. Western blot of SMAD1 expression in A-549.

Western blot

SMAD1 monoclonal antibody (M02), clone 1D3 Western blot of SMAD1 expression in IMR-32.
SMAD1 monoclonal antibody (M02), clone 1D3 Western blot of SMAD1 expression in IMR-32.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SMAD1 is approximately 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SMAD1 is approximately 3 ng/ml as a capture antibody.
Requested From: 
Date Requested: 4/19/2018
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