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Anti-SMAD1 Antibody (clone 1D3) LS-C133298

Catalog	Size	Price
LS-C133298-100	100 µg (0.5 mg/ml)	Unavailable

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Antibody:	Mouse Monoclonal (IgG1) to Human SMAD1
Reactivity:	Human, Mouse, Rat
Application:	ICC, Western blot, Immunoprecipitation, Flow Cytometry
Format:	Unconjugated

Anti-SMAD1 Antibody (N-Terminus) LS-C80224

Antibody:	Rabbit Polyclonal (IgG) to Human SMAD1
Reactivity:	Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila, Insect
Application:	IHC - Paraffin, Western blot
Format:	Unconjugated

Anti-SMAD1 Antibody (phospho-Ser206) LS-C154249

Antibody:	Rabbit Polyclonal (IgG) to Human SMAD1
Reactivity:	Human, Mouse, Rat, Dog
Application:	Western blot, Immunoprecipitation, ELISA
Format:	Unconjugated

Anti-SMAD1 Antibody (aa153-202) IHC-plus™ LS-B9239

Antibody:	Rabbit Polyclonal (IgG) to Human SMAD1
Reactivity:	Human, Mouse
Application:	IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Format:	Unconjugated

Anti-SMAD1 Antibody (phospho-Ser187) LS-C352485

Antibody:	Rabbit Polyclonal to Human SMAD1
Reactivity:	Human, Mouse, Rat, Sheep, Chicken
Application:	IHC - Paraffin, Western blot, Immunoprecipitation
Format:	Unconjugated

Antibody:	Mouse Monoclonal [clone 1D3] (IgG1,k) to Human SMAD1
Reactivity:	Human
Application:	Immunofluorescence, Western blot, ELISA
Format:	Unconjugated

Details

Target:	Human SMAD1
Host:	Mouse
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	IgG1,k Monoclonal [1D3]
Conjugations:	Unconjugated
Purification:	Purified from ascites by Protein A
Modifications:	Unmodified

Applications

Approved:

Immunofluorescence (10 µg/ml)
Western blot
ELISA

Failed:

IHC - Paraffin

Specificity and Use

Immunogen:	SMAD1 antibody was raised against sMAD1 (NP_005891, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity:	Human SMAD1
Usage:	Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.

Packaging

Presentation:	PBS, pH 7.2
Storage:	Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Restrictions:	For research use only.

About SMAD1

Uniprot: Q15797 NCBI: NM_005900 NP_005891.1

Synonyms: SMAD1 Antibody, BSP1 Antibody, BSP-1 Antibody, JV4-1 Antibody, MADH1 Antibody, Mothers against DPP homolog 1 Antibody, JV41 Antibody, SMAD family member 1 Antibody, TGF-beta signaling protein 1 Antibody, MADR1 Antibody, HSMAD1 Antibody, MAD homolog 1 Antibody, Mad-related protein 1 Antibody, SMAD 1 Antibody

Description: SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses.

(More About SMAD1)

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Immunofluorescence

Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody (M02), clone 1D3.

Western blot

SMAD1 monoclonal antibody (M02), clone 1D3. Western blot of SMAD1 expression in MES-SA/Dx5.

Western blot

SMAD1 monoclonal antibody (M02), clone 1D3. Western blot of SMAD1 expression in A-549.

Western blot

SMAD1 monoclonal antibody (M02), clone 1D3 Western blot of SMAD1 expression in IMR-32.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SMAD1 is approximately 3 ng/ml as a capture antibody.

Requested From:
Date Requested: 6/23/2017

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