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Antibodies > SMAD1 > LS-C344277
 
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Catalog Number Size Price
LS-C344277-400 400 µl $393 
SMAD1 Antibody - Immunohistochemical of paraffin-embedded H. skeletal muscle section using SMAD1 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
SMAD1 Antibody - Immunohistochemical of paraffin-embedded H. heart section using SMAD1 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
SMAD1 Antibody - Fluorescent image of MCF-7 cells stained with SMAD1 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
SMAD1 Antibody - Western blot of lysates from HT-1080, HUVEC, mouse C2C12 cell line and rat liver tissue lysate (from left to right) with SMAD1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
SMAD1 Antibody - Immunohistochemical of paraffin-embedded H. skeletal muscle section using SMAD1 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
SMAD1 Antibody - Immunohistochemical of paraffin-embedded H. heart section using SMAD1 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
SMAD1 Antibody - Fluorescent image of MCF-7 cells stained with SMAD1 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
SMAD1 Antibody - Western blot of lysates from HT-1080, HUVEC, mouse C2C12 cell line and rat liver tissue lysate (from left to right) with SMAD1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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Polyclonal Rabbit anti‑Human SMAD1 Antibody (aa163‑196, IHC, IF, WB) LS‑C344277

Polyclonal Rabbit anti‑Human SMAD1 Antibody (aa163‑196, IHC, IF, WB) LS‑C344277

Submit Review LSBio Guarantee
Antibody:
SMAD1 Rabbit anti-Human Polyclonal (aa163-196) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C344277-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SMAD1 Rabbit anti-Human Polyclonal (aa163-196) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD1 antibody LS-C344277 is an unconjugated rabbit polyclonal antibody to SMAD1 (aa163-196) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human SMAD1
Synonyms
SMAD1 | BSP1 | BSP-1 | JV4-1 | MADH1 | Mothers against DPP homolog 1 | JV41 | SMAD family member 1 | TGF-beta signaling protein 1 | MADR1 | HSMAD1 | MAD homolog 1 | Mad-related protein 1 | SMAD 1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa163-196
Specificity
This SMAD1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 163-196 amino acids from the Central region of human SMAD1.
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence (1:25)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD1
Q15797 NM_005900 NP_005891.1

Publications (0)

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Featured Products

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SMAD1 Antibody LS‑C75853
Species: Human, Mouse, Rat
Applications: ICC, Western blot, Immunoprecipitation, Flow Cytometry
SMAD1 Antibody - Anti-SMAD1 Antibody - Western Blot. Western blot of Affinity Purified anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37?C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (B501-0500) and reacted for 1 hour at room temperature. Anti-beta actin (p/n LS-B323) staining was used as a loading control. The membrane was washed and reacted with a 1:3000 dilution HRP-conjugated a-Rabbit IgG (p/n LS-C60865) for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.
SMAD1 Antibody LS‑C154249
Species: Human, Mouse, Rat, Dog
Applications: Western blot, Immunoprecipitation, ELISA
SMAD1 Antibody - Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
SMAD1 Antibody (clone 1D3) LS‑C133298
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
SMAD1 Antibody - SMAD1 antibody SMAD1(SMAD family member 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human skin. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SMAD1 Antibody (N‑Terminus) LS‑C80224
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
SMAD1 Antibody - A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SMAD1 Antibody (C‑Terminus) LS‑C31108
Species: Human
Applications: Western blot
SMAD1 Antibody - Immunohistochemical analysis of SMAD1 (pS187) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
SMAD1 Antibody (phospho‑Ser187) LS‑C352485
Species: Human, Mouse, Rat, Sheep, Chicken
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
See all SMAD1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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