SLIT1 antibody LS-B721 is an unconjugated rabbit polyclonal antibody to SLIT1 from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500 - 1:2000)
- ELISA (1:10000 - 1:50000)
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SLIT1 antibody was raised against synthetic peptide from mouse SLIT1.
Immunohistochemistry: LS-B721 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B721 was determined to be 5 ug/ml.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.