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Anti-SLIM / FHL1 Antibody (aa261-272) IHC-plus™ LS-B2495

Note: This antibody replaces LS-C54838, LS-C66025


Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular SLIM / FHL1 Antibodies

Anti-SLIM / FHL1 Antibody (aa207-256) LS-C30688
Rabbit Polyclonal (IgG) to Human SLIM / FHL1
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Sheep
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-SLIM / FHL1 Antibody (aa261-272) IHC-plus™ LS-B2495
Goat Polyclonal to Human SLIM / FHL1
Human, Mouse, Rat, Hamster
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-SLIM / FHL1 Antibody (aa51-100) IHC-plus™ LS-B3484
Rabbit Polyclonal (IgG) to Human SLIM / FHL1
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Goat Polyclonal to Human SLIM / FHL1
Human, Mouse, Rat, Hamster
IHC - Paraffin, Western blot, ELISA


Human SLIM / FHL1
Human, Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
Monkey, Bovine, Dog, Horse, Pig, Rabbit, Sheep, Xenopus (at least 90% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:32000) & (0.05 - 0.3 µg/ml)
  • ELISA (1:32000)

Specificity and Use

SLIM / FHL1 antibody was raised against synthetic peptide CNKRFVFHNEQVY from an internal region of human FHL1 (NP_001440.1; NP_001153171.1). Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%); Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Xenopus (92%).
LS-E25772 - Lyophilized - 100 µg - $145.00
Human FHL1. This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.
Immunohistochemistry: LS-B2495 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2495 was determined to be 2.5 ug/ml.


Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

Publications (1)

Adenylyl cyclase 6 deletion reduces left ventricular hypertrophy, dilation, dysfunction, and fibrosis in pressure-overloaded female mice. Tang T, Lai NC, Hammond HK, Roth DM, Yang Y, Guo T, Gao MH. Journal of the American College of Cardiology. 2010 55:1476-86. [PubMed:20359598] [PMC:PMC3081694]

About SLIM / FHL1

Q13642 NM_001449 NP_001440.2

FHL1 Antibody, BA535K18.1 Antibody, FHL1B Antibody, FHL1A Antibody, FLH1A Antibody, Four and a half LIM domains 1 Antibody, FHL-1 Antibody, KYOT Antibody, Skeletal muscle LIM-protein 1 Antibody, SLIM-1 Antibody, Skeletal muscle LIM-protein1 Antibody, SLIMMER Antibody, XMPMA Antibody, KYO-T Antibody, LIM protein SLIMMER Antibody, SLIM Antibody, SLIM1 Antibody

SLIM / FHL1 is a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy.


Anti-FHL1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2495 concentration 5 ug/ml.

Western blot

Western blot
Antibody staining (0.05 ug/ml) of Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Requested From: United States
Date Requested: 10/27/2016

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