Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey (tested or 100% immunogen sequence identity)
Mouse, Bat, Bovine, Hamster (at least 90% immunogen sequence identity)
IHC - Paraffin (10 µg/ml)
Specificity and Use
SLC5A9 / SGLT4 antibody was raised against synthetic 15 amino acid peptide from C-terminus of human SLC5A9. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Gibbon, Mouse, Bat, Bovine, Hamster, Elephant (93%); Turkey, Chicken (80%).
Human SLC5A9. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except AFAP1L2 (60%).
Immunohistochemistry: LS-A2788 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A2788 was determined to be 10 ug/ml.
PBS, less than 0.1% sodium azide.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Identification of a novel sodium-dependent fructose transport activity in the hepatopancreas of the Atlantic lobster Homarus americanus. Sterling KM, Cheeseman CI, Ahearn GA. The Journal of experimental biology. 2009 Jun;212:1912-20. [Full Text Article]