Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
Western blot (1:1000 - 1:5000)
ELISA (1:10000 - 1:50000)
SLC4A9 / AE4 antibody was raised against a 15 AA Peptide sequence near the cytoplasmic C-terminus of human AE4 (1). The peptide was coupled to KLH.
The 15 AA human control peptide is 100% conserved in rabbit AE4. The sequence is also the same in proteins listed as human NBC5 (gene accession # AB032762, 657 aa) and NBC (gene accession # AF313465, 990 aa; XM_038736, 959 aa). No significant sequence homology of is detected with other AE1-3 or other transporters. Antibody cross-reactivity in various species is not known. Control peptide, because of its small size (<3kD), is not recommended for western. It should be used as a positive control in ELISA, dot blot or as blocking peptide (use 5-10 ug of control peptide per 1 ug IgG or 1 ul of antiserum) to confirm antibody specificity.
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000.
PBS, 0.05% Sodium Azide, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.