Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Rabbit (tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
Western blot (1:1000 - 1:5000), ELISA (1:10000 - 1:50000)
Specificity and Use
SLC4A9 / AE4 antibody was raised against a 15 AA Peptide sequence near the cytoplasmic C-terminus of human AE4 (1). The peptide was coupled to KLH.
The 15 AA human control peptide is 100% conserved in rabbit AE4. The sequence is also the same in proteins listed as human NBC5 (gene accession # AB032762, 657 aa) and NBC (gene accession # AF313465, 990 aa; XM_038736, 959 aa). No significant sequence homology of is detected with other AE1-3 or other transporters. Antibody cross-reactivity in various species is not known. Control peptide, because of its small size (<3kD), is not recommended for western. It should be used as a positive control in ELISA, dot blot or as blocking peptide (use 5-10 ug of control peptide per 1 ug IgG or 1 ul of antiserum) to confirm antibody specificity.
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000.
PBS, 0.05% sodium azide, 40% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.