NBC1 antibody LS-C16720 is an unconjugated rabbit polyclonal antibody to human NBC1 (SLC4A4). Validated for ELISA and WB.
Human SLC4A4 / NBC1
(tested or 100% immunogen sequence identity)
- Western blot (1 - 10 µg/ml)
- ELISA (1:10)
SLC4A4 / NBC1 antibody was raised against a 20-aa peptide sequence within the cytoplasmic, N-terminus of human NBC1 (1) was used to generate anti-NBC1. The peptide was coupled with KLH (carrier protein).
Human sequence is 100% conserved in mouse, rat, human and rabbit NBC1/PSLC4A4/kNBC and 75% in frog NBC1. This region is also shared 100% with pNBC/ PSLC4A5. Therefore, antibodies will recognize both the kidney and pancreatic/heart isoforms. No significant sequence homology of was found with NBC2 or NBC3.
Suitable for use in ELISA and Western Blot. ELISA: 1:10K-1:100K using 50-100 ng of control peptide/well. Western Blot: 1-10 ug/ml using ECL.
100 mM Tris, pH 7.5, 0.05% Sodium Azide, 40% Glycerol, 0.2% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.