Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Western blot (1 - 10 µg/ml)
SLC4A4 / NBC1 antibody was raised against a 20-aa peptide sequence within the cytoplasmic, N-terminus of human NBC1 (1) was used to generate anti-NBC1. The peptide was coupled with KLH (carrier protein).
Human sequence is 100% conserved in mouse, rat, human and rabbit NBC1/PSLC4A4/kNBC and 75% in frog NBC1. This region is also shared 100% with pNBC/ PSLC4A5. Therefore, antibodies will recognize both the kidney and pancreatic/heart isoforms. No significant sequence homology of was found with NBC2 or NBC3.
Suitable for use in ELISA and Western Blot. ELISA: 1:10K-1:100K using 50-100 ng of control peptide/well. Western Blot: 1-10 ug/ml using ECL.
Electrogenic sodium/bicarbonate cotransporter with a Na+:HCO3- stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.