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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SLC4A4 / NBC1 Antibody (N‑Terminus) LS‑C16720
NBC1 antibody LS-C16720 is an unconjugated rabbit polyclonal antibody to human NBC1 (SLC4A4). Validated for ELISA and WB.
Antibody
Catalog
Size
Price
LS-C16720-100
100 µg
$755

Popular SLC4A4 / NBC1 Products

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Immunohistochemical analysis of NBC1 staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

SLC4A4 / NBC1 Antibody (N-Terminus) for ELISA, WB/Western LS-C16720

Specifications

Target
Human SLC4A4 / NBC1
Synonyms
SLC4A4, HhNMC, HNBC1, KNBC, KNBC1, Na+-HCO-3 cotransporter, NBC2, NBCE1, NBC1, PNBC1, Na(+)/HCO3(-) cotransporter, PNBC, HhNBC, NBC
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1 - 10 µg/ml)
  • ELISA (1:10)
Immunogen
SLC4A4 / NBC1 antibody was raised against a 20-aa peptide sequence within the cytoplasmic, N-terminus of human NBC1 (1) was used to generate anti-NBC1. The peptide was coupled with KLH (carrier protein).
Epitope
N-Terminus
Specificity
Human sequence is 100% conserved in mouse, rat, human and rabbit NBC1/PSLC4A4/kNBC and 75% in frog NBC1. This region is also shared 100% with pNBC/ PSLC4A5. Therefore, antibodies will recognize both the kidney and pancreatic/heart isoforms. No significant sequence homology of was found with NBC2 or NBC3.
Usage
Suitable for use in ELISA and Western Blot. ELISA: 1:10K-1:100K using 50-100 ng of control peptide/well. Western Blot: 1-10 ug/ml using ECL.
Presentation
100 mM Tris, pH 7.5, 0.05% Sodium Azide, 40% Glycerol, 0.2% BSA
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SLC4A4 / NBC1
Electrogenic sodium/bicarbonate cotransporter with a Na+:HCO3- stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. Q9Y6R1 NM_003759 NP_003750.1


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Requested From: United States
Date Requested: 10/15/2018

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