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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SLC4A1 / Band 3 / AE1 Antibody (N‑Terminus) LS‑C11864
AE1 antibody LS-C11864 is an unconjugated rabbit polyclonal antibody to rat AE1 (SLC4A1 / Band 3). Validated for ELISA, IHC and WB.
Antibody
Catalog
Size
Price
LS-C11864-100
100 µg
$755

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Applications: Western blot, ELISA

Product Description

AE1 antibody LS-C11864 is an unconjugated rabbit polyclonal antibody to rat AE1 (SLC4A1 / Band 3). Validated for ELISA, IHC and WB.

Specifications

Target
Rat SLC4A1 / Band 3 / AE1
Synonyms
SLC4A1, Anion exchanger 1, Anion exchanger-1, AE1, Anion exchange protein 1, Band 3, Band 3 anion transport protein, BND3, CD233, DI, EPB3, AE 1, Froese blood group, FR, RTA1A, SW, Swann blood group, WD1, WR, Wright blood group, WD, CD233 antigen, EMPB3, Waldner blood group
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (2 - 10 µg/ml)
  • Western blot (1 - 10 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
An 20 AA Peptide sequence near the N-terminus of rat AE-1 (1). The peptide was coupled to KLH.
Epitope
N-Terminus
Specificity
The 20 AA rat control peptide is 90% conserved in mouse, 75% in human and 70% in bovine AE1. No significant sequence homology is detected with other AE or other proteins.
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1-10 ug/ml using ECL. ELISA: 0.5-1 ug/ml Control peptide can be used to coat ELISA plates at 1 ug/ml. Immunohistochemistry: 2-10 ug/ml.
Presentation
Lyophilized from PBS, pH 7.4, 0.1% BSA
Reconstitution
Sterile ddH2O or PBS
Storage
Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.
Restrictions
For research use only.
About SLC4A1 / Band 3 / AE1
SLC4A1 / Band 3 / AE1 is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. P02730 NM_000342 NP_000333.1


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Requested From: United States
Date Requested: 10/21/2018

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