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Anti-SLC4A1 / Band 3 / AE1 Antibody LS-C11866

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Mouse Monoclonal (IgG2a) to Human SLC4A1 / Band 3 / AE1
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human SLC4A1 / Band 3 / AE1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Ascites

Applications

Immunofluorescence, Western blot (1:1000 - 1:5000), ELISA (1:10000 - 1:50000)

Specificity and Use

SLC4A1 / Band 3 / AE1 antibody was raised against purified human Anion Exchanger 1 (AE1, Band3).
Recognizes human Anion Exchanger 1 (AE-1, Band3). Does not recognize non-erythroid band-3 from human fibroblasts. Species cross-reactivity: Does not recognize band-3 from equine, mouse, bovine, porcine, guinea pig, canine or mouse erythrocytes.
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000. AE1 is ~90-100kD protein. It aggregates easily forming dimers and multimers. Aggregation may be avoided if membranes are prepared in N-ethylmaleimide. It may also be proteolytically processed to smaller fragments of 60, 40 and 20kD. ELISA: 1:10000-1:50000.

Packaging

PBS, 0.05% sodium azide, 40% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SLC4A1 / Band 3 / AE1

P02730 NM_000342 NP_000333.1

SLC4A1 Antibody, Anion exchanger 1 Antibody, Anion exchanger-1 Antibody, AE1 Antibody, Anion exchange protein 1 Antibody, Band 3 Antibody, Band 3 anion transport protein Antibody, BND3 Antibody, CD233 Antibody, DI Antibody, EPB3 Antibody, AE 1 Antibody, Froese blood group Antibody, FR Antibody, RTA1A Antibody, SW Antibody, Swann blood group Antibody, WD1 Antibody, WR Antibody, Wright blood group Antibody, WD Antibody, CD233 antigen Antibody, EMPB3 Antibody, Waldner blood group Antibody

SLC4A1 / Band 3 / AE1 is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin.

Requested From: United States
Date Requested: 9/30/2016

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