Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
SLC4A1, Anion exchanger 1, Anion exchanger-1, AE1, Anion exchange protein 1, Band 3, Band 3 anion transport protein, BND3, CD233, DI, EPB3, AE 1, Froese blood group, FR, RTA1A, SW, Swann blood group, WD1, WR, Wright blood group, WD, CD233 antigen, EMPB3, Waldner blood group
(tested or 100% immunogen sequence identity)
Western blot (1:1000 - 1:5000)
ELISA (1:10000 - 1:50000)
Purified human Anion Exchanger 1 (AE1, Band3).
Recognizes human Anion Exchanger 1 (AE-1, Band3). Does not recognize non-erythroid band-3 from human fibroblasts. Species cross-reactivity: Does not recognize band-3 from equine, mouse, bovine, porcine, guinea pig, canine or mouse erythrocytes.
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000. AE1 is ~90-100kD protein. It aggregates easily forming dimers and multimers. Aggregation may be avoided if membranes are prepared in N-ethylmaleimide. It may also be proteolytically processed to smaller fragments of 60, 40 and 20kD. ELISA: 1:10000-1:50000.
PBS, 0.05% Sodium Azide, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
SLC4A1 / Band 3 / AE1 is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin.