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Catalog Number Size Price
LS-C407650-10 10 µg $318 
LS-C407650-100 100 µg $470 
SLC4A1 / Band 3 / AE1 Antibody - Band 3 antibody IHC-paraffin. IHC(P): Mouse Kidney Tissue.
SLC4A1 / Band 3 / AE1 Antibody - Band 3 antibody IHC-frozen. IHC(F): Human Placenta Tissue.
SLC4A1 / Band 3 / AE1 Antibody - Band 3 antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.
SLC4A1 / Band 3 / AE1 Antibody - Band 3 antibody IHC-paraffin. IHC(P): Human Placenta Tissue.
SLC4A1 / Band 3 / AE1 Antibody - IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Band 3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SLC4A1 / Band 3 / AE1 Antibody - IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Band 3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SLC4A1 / Band 3 / AE1 Antibody - Band 3 antibody Western blot. All lanes: Anti Band 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Lane 3: Rat Liver Tissue Lysate at 50 ug. Lane 4: Rat Spleen Tissue Lysate at 50 ug. Lane 5: Human Placenta Tissue Lysate at 50 ug. Predicted band size: 102 kD. Observed band size: 102 kD.
SLC4A1 / Band 3 / AE1 Antibody - Band 3 antibody IHC-paraffin. IHC(P): Mouse Kidney Tissue.
SLC4A1 / Band 3 / AE1 Antibody - Band 3 antibody IHC-frozen. IHC(F): Human Placenta Tissue.
SLC4A1 / Band 3 / AE1 Antibody - Band 3 antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.
SLC4A1 / Band 3 / AE1 Antibody - Band 3 antibody IHC-paraffin. IHC(P): Human Placenta Tissue.
SLC4A1 / Band 3 / AE1 Antibody - IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Band 3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SLC4A1 / Band 3 / AE1 Antibody - IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Band 3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SLC4A1 / Band 3 / AE1 Antibody - Band 3 antibody Western blot. All lanes: Anti Band 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Lane 3: Rat Liver Tissue Lysate at 50 ug. Lane 4: Rat Spleen Tissue Lysate at 50 ug. Lane 5: Human Placenta Tissue Lysate at 50 ug. Predicted band size: 102 kD. Observed band size: 102 kD.
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Polyclonal Rabbit anti‑Human SLC4A1 / Band 3 / AE1 Antibody (aa28‑365, IHC, WB) LS‑C407650

Polyclonal Rabbit anti‑Human SLC4A1 / Band 3 / AE1 Antibody (aa28‑365, IHC, WB) LS‑C407650

Antibody:
SLC4A1 / Band 3 / AE1 Rabbit anti-Human Polyclonal (aa28-365) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407650-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SLC4A1 / Band 3 / AE1 Rabbit anti-Human Polyclonal (aa28-365) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AE1 antibody LS-C407650 is an unconjugated rabbit polyclonal antibody to AE1 (SLC4A1 / Band 3) (aa28-365) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human SLC4A1 / Band 3 / AE1
Synonyms
SLC4A1 | Anion exchanger 1 | Anion exchanger-1 | AE1 | Anion exchange protein 1 | Band 3 | Band 3 anion transport protein | BND3 | CD233 | DI | EPB3 | AE 1 | Froese blood group | FR | RTA1A | SW | Swann blood group | WD1 | WR | Wright blood group | WD | CD233 antigen | EMPB3 | Waldner blood group
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human Band 3 (Position: E28-N365). Human Band 3 shares 75.7% and 74.5% amino acid (aa) sequence identity with mouse and rat Band 3, respectively.
Epitope
aa28-365
Specificity
Detected in erythrocytes (at protein level). Erythrocytes. .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • IHC - Frozen (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC4A1 / Band 3 / AE1
P02730 NM_000342 NP_000333.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/19/2024