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Anti-SLC35A5 Antibody (aa36-85) IHC-plus™ LS-B3555

Note: This antibody replaces LS-C80627
Catalog Size Price
LS-B3555-50 50 µg Unavailable

Related Products

3 SLC35A5 Antibodies

Most Popular SLC35A5 Antibodies

Anti-SLC35A5 Antibody (aa36-85) IHC-plus™ LS-B3555
Rabbit Polyclonal (IgG) to Human SLC35A5
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SLC35A5 Antibody (aa36-85, FITC) LS-C445881
Rabbit Polyclonal (IgG) to Human SLC35A5
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot
FITC Conjugated
Immunohistochemistry Image
Anti-SLC35A5 Antibody (aa36-85, Biotin) LS-C445882
Rabbit Polyclonal (IgG) to Human SLC35A5
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot
Biotin Conjugated
Immunohistochemistry Image
Anti-SLC35A5 Antibody (aa36-85, HRP) LS-C445883
Rabbit Polyclonal (IgG) to Human SLC35A5
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot
HRP Conjugated
Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal (IgG) to Human SLC35A5
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SLC35A5
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Rabbit, Chicken (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

SLC35A5 antibody was raised against synthetic peptide located between aa36-85 of human SLC35A5 (Q9BS91, NP_060415). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig, Platypus (100%); Rabbit, Turkey, Zebra finch, Chicken, Lizard (92%); Opossum (85%); Zebrafish (84%).
LS-E16163 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3555. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa36-85
Human SLC35A5
Immunohistochemistry: LS-B3555 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3555 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SLC35A5

Q9BS91 NM_017945 NP_060415.1

SLC35A5 Antibody


Immunohistochemistry

Immunohistochemistry
Anti-SLC35A5 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3555 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-SLC35A5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3555 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 6/28/2017

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