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Anti-SLC2A5 / GLUT5 Antibody (N-Terminus) LS-C15416

Ordering

Wt. Vol. Conc. Price
50 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular SLC2A5 / GLUT5 Antibodies

Anti-SLC2A5 / GLUT5 Antibody LS-C179227
Rabbit Polyclonal (IgG) to Human SLC2A5 / GLUT5
Human
IHC - Paraffin, ICC, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-SLC2A5 / GLUT5 Antibody (clone 20E1) LS-C114673
Mouse Monoclonal [clone 20E1] (IgG1) to Human SLC2A5 / GLUT5
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-SLC2A5 / GLUT5 Antibody (aa232-251) LS-C312888
Rabbit Polyclonal (IgG) to Mouse SLC2A5 / GLUT5
Mouse
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal (IgG) to Human SLC2A5 / GLUT5
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human SLC2A5 / GLUT5
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Immunofluorescence
  • Western blot (1 - 10 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

SLC2A5 / GLUT5 antibody was raised against a cytoplasmic 12 AA peptide sequence near the C-terminus of human glucose/fructose transporter (Glut-5) (1). A cysteine has been added at the NH2 terminus for coupling to KLH. The peptide was coupled to KLH.
N-Terminus
Human GT52 sequences shows about 61% homology with rat Glut-5 (8/13 AA). For rat Glut-5, we suggest the use of antibodies that are made to a peptide from rat Glut-5. Antibody cross-reactivity in various species is not established. Control peptide, because of its low mol size (<3kD), is not recommended for Western. It should be used in ELISA, Dot Blot and for antibody blocking to confirm specificity of antibodies.
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Western Blot: 1-10 ug/ml (ECL). Rat glut-5 is ~60kD in rat jejunum membranes that is slightly larger that that reported for human glut-5 (50-55kD). As opposed to human tissues, rat glut-5 mRNA has not been detected in rat testis, adipose tissues and skeletal muscle. In rat kidney, a protein of ~42kD was detected. ELISA: 0.5-1 ug/ml. Control peptide can be used to coat ELISA plates at 1 ug/ml.

Packaging

PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About SLC2A5 / GLUT5

P22732 NM_003039 NP_003030.1

SLC2A5 Antibody, Fructose transporter Antibody, Glucose transporter 5 Antibody, GLUT-5 Antibody, GLUT5 Antibody

SLC2A5/GLUT5 belongs to a family of glucose/fructose transport proteins that exhibit 45-65% amino acid homology. GLUT 5 functions mainly as a fructose transporter and is thought to be largely responsible for the uptake of fructose from the lumen of the small intestine. Its' expression is induced in mice exposed to high fructose diets. GLUT5 is also responsible for the uptake of fructose by spermatozoa.

Requested From: United States
Date Requested: 12/9/2016

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