Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Rat, Hamster
(tested or 100% immunogen sequence identity)
Mouse, Cat (at least 90% immunogen sequence identity)
Western blot (1:1000 - 1:5000)
ELISA (1:10000 - 1:50000)
SLC20A2 / PIT2 antibody was raised against a 19 AA synthetic peptide within the 4th putative cytoplasmic domain of Human PiT-2/GLVR-2 (1) was synthesized, conjugated to KLH.
The peptide is 100% conserved in rat, hamster and 94% in mouse, feline PiT-2/GLVR-2. No significant sequence homology is observed with PiT-1 or NaPi-Type I, -2, or IV transporters. Antibody cross-reactivity in various species is not established.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000. A band of 95-110kD has been detected in brush border membrane. ELISA: 1:10000-1:50000. Control peptide can be used to coat ELISA plates at 1 ug/ml.
PBS, 0.05% Sodium Azide, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Sodium-phosphate symporter which seems to play a fundamental housekeeping role in phosphate transport by absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. In vitro, sodium-dependent phosphate uptake is not siginificantly affected by acidic and alkaline conditions, however sodium-independent phosphate uptake occurs at acidic conditions.