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Anti-SLC18A3 / VACHT Antibody (C-Terminus) LS-C18492

Catalog Size Price
LS-C18492-20 20 µg Unavailable

Most Popular SLC18A3 / VACHT Antibodies

Anti-SLC18A3 / VACHT Antibody (C-Terminus) LS-C18492
Guinea pig Polyclonal (IgG) to Rat SLC18A3 / VACHT
Rat
IHC - Frozen, Western blot
Unconjugated
Anti-SLC18A3 / VACHT Antibody (C-Terminus) LS-C18494
Chicken Polyclonal (IgY) to Mouse SLC18A3 / VACHT
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Western blot, ELISA
Unconjugated
Anti-SLC18A3 / VACHT Antibody (clone S6-38) LS-C150468
Mouse Monoclonal [clone S6-38] (IgG1)
Human, Mouse, Rat
IHC
Unconjugated
Anti-SLC18A3 / VACHT Antibody LS-C179239
Rabbit Polyclonal (IgG) to Human SLC18A3 / VACHT
Human
IHC - Paraffin, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-SLC18A3 / VACHT Antibody LS-C184160
Goat Polyclonal (IgG) to Monkey SLC18A3 / VACHT
Monkey, Human, Mouse, Rat
IHC, ICC
Unconjugated

100% Guaranteed
Guinea pig Polyclonal (IgG) to Rat SLC18A3 / VACHT
Rat
IHC - Frozen, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Rat SLC18A3 / VACHT
Guinea pig
Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Affinity purified
Unmodified

Applications

  • IHC - Frozen
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

SLC18A3 / VACHT antibody was raised against synthetic peptide corresponding to the C-terminus of the predicted rat VAChT protein.
C-Terminus
Recognizes VAChT. Species cross-reactivity: Rat.
Suitable for use in Immunohistochemistry and Western Blot. Immunohistochemistry (4% paraformaldehyde fixed tissue): 1:500-1:1500, staining was strong in the inner retina where cholinergic processes are located and weak in cholinergic cell bodies. There was no background staining noted. In the CNS staining was strong in fibers and stains cholinergic cell bodies well. Sections were cut at 15 um with a cryostat or 30 um with a sliding microtome. Western Blot: Reacted with a single band in rat brain homogenates. This band corresponded to the size of the predicted rVAChT. There was no staining of blots incubated with the antibody that had been preabsorbed with the peptide used for antibody production.

Packaging

Lyophilized.
20 µl deionized water
Store lyophilized at 4°C for short term only. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
For research use only.

About SLC18A3 / VACHT

Q16572 NM_003055 NP_003046.2

SLC18A3 Antibody, HVAChT Antibody, VACHT Antibody, RVAChT Antibody, Vesicular ACh transporter Antibody

SLC18A3 / VACHT is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.

Requested From: 
Date Requested: 6/26/2017

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